We narrowed to 12,266 results for: cel.2;

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pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Article

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Expression

Bacterial

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa1-149

Plasmid

#73410

Purpose

Expresses HA tagged aa1-149 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-149 only of AKT1

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-Akt1-aa120-433

Plasmid

#73411

Purpose

Expresses HA tagged aa120-433 AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 120-433 of AKT1

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA-IgKappa-myc-dL5- 2XG4S-GPI

Plasmid

#153308

Purpose

Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)

Depositor

Marcel Bruchez

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

IgKappa-myc-dL5-2XG4S-GPI

Tags

myc-dL5

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

Plasmid

#132685

Purpose

AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 2 (Cndp2 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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hCEP120-Myc

Plasmid

#50381

Purpose

Tet-On inducible expression of Myc-tagged hCEP120

Depositor

Tang Tang

Article

Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

Insert

Human CEP120 (CEP120 Human)

Tags

Myc

Expression

Mammalian

Mutation

This hCEP120 contains 4 SNPs (rs6876883, rs659544…

Promoter

CMV-2xTetO

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

Plasmid

#197893

Purpose

Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

mCherry

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai-IRES-GFP

Plasmid

#72893

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe HA-∆Raf1(S642A):ER* puro

Plasmid

#72572

Purpose

Expresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

Raf1 (RAF1 Human)

Use

Retroviral

Tags

ER* and HA

Expression

Mammalian

Mutation

amino acids 306-648, serine 642 mutated to alanine

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-Akt1-aa1-408

Plasmid

#73412

Purpose

Expresses HA tagged aa1-408 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-408 of AKT1

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
dmBACCS2NS-IRES-dOrai-IRES-GFP

Plasmid

#72895

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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CIB-VP16 (B1014)

Plasmid

#92036

Purpose

Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Fusion of plant CIB1 (intact NLS) with VP16 activ…

Expression

Mammalian

Promoter

T7

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3Basic-miR126-EGFL7-Promoter

Plasmid

#32244

Depositor

Charles Lowenstein

Article

Harris et al Arterioscler Thromb Vasc Biol. 2010 Oct;

Insert

miR-126 Promoter 5' UTR (MIR126, EGFL7 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Promoter region 5' UTR

Promoter

5' UTR

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pYL214

Plasmid

#171031

Purpose

CRISPR plasmids encodes Cas9 and a sgRNA targeting EZH2 exon 2 - intron 2 junction (sequence: GCAGACGAGCTGATGAAGTAA)

Depositor

Thomas Cech

Article

Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

Insert

EZH2 (EZH2 Human)

Use

CRISPR

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-FGF21

Plasmid

#182407

Purpose

Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-fgf21 (FGF21 Human)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(Luc)-GFP

Plasmid

#197887

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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HA-VHL C162F-pRc/CMV

Plasmid

#22042

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 162 from Cysteine to …

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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HA-VHL L158S-pRc/CMV

Plasmid

#22041

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant converting aa 162 from Cysteine to P…

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-mCherry

Plasmid

#72898

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCW57.1-pDUXChd:hDUX4ctd-ca

Plasmid

#198237

Purpose

Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

DUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 Human, S. scrofa (porcine))

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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