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C122-E17: mPlum

Plasmid

#162897

Purpose

Standard Gateway entry clone encoding mPlum fluorescent protein

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Plum fluorescent protein

Use

Synthetic Biology

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pN23-LIC-His-FLAG

Plasmid

#165478

Purpose

pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Publication

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Bacterial

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GGDestX1-Amp

Plasmid

#157652

Purpose

Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

Depositor

Karmella Haynes

Publication

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Cell-free expression (e. coli sigma-70)

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-686 (attR4-attR2)

Plasmid

#161889

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

myc eGFP 3C

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSM04

Plasmid

#139046

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (no STOP codon)

Expression

Worm

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK130

Plasmid

#110584

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 promoter with lac operator and RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCB6-BHAPI

Plasmid

#99350

Purpose

BHA-PI (gpi anchored X:31 HA)

Depositor

Judith White

Publication

Armstrong et al J Cell Biol. 2000 Oct 16;151(2):425-37.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gpi anchored X:31 HA

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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LI D-E C-NLS c2

Plasmid

#54428

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

NLS of SV40 large T antigen

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D destab GFP c1

Plasmid

#54454

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

destablized GFP

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBSII-NLS-mCherry(AM)

Plasmid

#244814

Purpose

In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mCherry-labeled Nuclear Localization Signal (NLS) Tag

Depositor

Frederic Strobl

Insert

mCherry

Use

In-vitro mrna synthesis

Tags

SV40 NLS

Mutation

coding sequence is optimized for the Honeybee

Promoter

T7

Available since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Cre-LPETG-6xHis

Plasmid

#237224

Purpose

Expresses LPETG-tagged Cre recombinase in bacteria

Depositor

Elliot Chaikof

Publication

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Cre-LPETG (cre Synthetic)

Tags

6xHis

Expression

Bacterial

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGLAST-DEST (JDW 1009)

Plasmid

#229822

Purpose

A single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAG43

Plasmid

#226736

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Publication

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MICU1 (targeting sequence) (MICU1 Human)

Use

Lentiviral

Tags

GFP11

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pGM265

Plasmid

#220179

Purpose

Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Publication

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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AA015

Plasmid

#216058

Purpose

Fragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFP

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

P2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA184

Plasmid

#216047

Purpose

Fragmid fragment: (C' terminus) for prime editing; using PEmax

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA406

Plasmid

#216070

Purpose

Fragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistance

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

P2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKE014

Plasmid

#207908

Purpose

GG entry for zCas9i expression

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9_introns_2xNLS-Pea3A(T) term

Use

CRISPR

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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