We narrowed to 9,039 results for: CAG

1. TLCV2-sgHPDL #9

   Plasmid

   #174166

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-SMCR8

   Plasmid

   #83440

   Purpose

   Expresses Cas9 and a gRNA targeting SMCR8

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human SMCR8 (SMCR8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-shCtnnd2.1-GFP

   Plasmid

   #209095

   Purpose

   GFP expressing shRNA targeting Ctnnd2 N-terminus

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   shCtnnd2.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuidPuro-hTP53_sgRNA-1

   Plasmid

   #88853

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

   Plasmid

   #222994

   Purpose

   Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCX-2A-2A

   Plasmid

   #74670

   Purpose

   based on pCX-C1 with F2A1/F2A2 inserted

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. wtTDP43tdTOMATOHA

   Plasmid

   #28205

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   HA tag and tdtomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB-GFP

   Plasmid

   #11155

   Purpose

   Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   Ubiquitin C promoter (UBC Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEFIRES-P-EGFP-ADRP

   Plasmid

   #87157

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   mCherry-R TTGGTCACCTTCAGCTTGG

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-WIPI2d-TEVcs-TSF

    Plasmid

    #171420

    Purpose

    For the purifcation of GFP- WIPI2d protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB025

    Plasmid

    #183092

    Purpose

    Expresses FnCas12a in Bacteroides and used for genome editing

    Depositor

    Lei Dai

    Article

    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

    Insert

    FnCas12a, gRNA, Promoter, HAB, TetR,
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1TDPGH023

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRISPR_HLA_class_I_1

    Plasmid

    #164986

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Gg 3kb Green opsin GFP

    Plasmid

    #72919

    Purpose

    green opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFP

    Depositor

    Joseph Corbo

    Article

    Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

    Insert

    chicken green opsin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TBP6.7 non-targeting gRNA lambda phage

    Plasmid

    #132547

    Purpose

    expresses gRNA for TBP-based CIRTS

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    gRNA TBP-based CIRTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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