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pEPkan-S2

Plasmid

#61601

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Publication

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRSII314

Plasmid

#35451

Depositor

Steven Haase

Publication

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast centromere vector

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-3xflagGREB1a

Plasmid

#112221

Purpose

mammalian expression of flag tagged GREB1a

Depositor

Craig Burd

Publication

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1a (GREB1 Human)

Tags

3x flag

Expression

Mammalian

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-mNeptune2.5

Plasmid

#104013

Purpose

Plasmid encoding mNeptune2.5

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mNeptune2.5

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (MSKGEE LIKENM… M11…

Promoter

proC

Available since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKAR2A

Plasmid

#20601

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKAR2A (PRKAR2A Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAAV_BiSSTe4_GCaMP6f

Plasmid

#213947

Purpose

AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

GCaMP6f

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_Empty

Plasmid

#221480

Purpose

Empty vector control for pLX307 overexpresion experiments

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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attB-GFP

Plasmid

#65521

Purpose

Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

Depositor

Sebastian Bultmann

Publication

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3

Plasmid

#17993

Depositor

Jan Kitajewski

Publication

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3 (WNT3 Human)

Use

Retroviral

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHR-mCherry-P2A-strepII-PPPCB

Plasmid

#67923

Purpose

Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

Depositor

Ron Vale

Publication

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBbS8c_ddCpf1_final

Plasmid

#212040

Purpose

Bacterial expression of the CRISPRi system for genome editing in E. coli

Depositor

Quanjiang Ji

Publication

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

ddCpf1

Use

CRISPR

Promoter

L-arabinose-inducible promoter PBAD

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CK(0.4)GW

Plasmid

#27226

Depositor

Pavel Osten

Publication

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

AAV

Tags

EGFP

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCM1.58

Plasmid

#21380

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pie-1 promoter (short) in pDONRP4P1R

Use

Gateway Cloning

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pGME

Plasmid

#71252

Purpose

luciferase reporter with GM-CSF promoter + 717 bp Bgl II GM-CSF enhancer

Depositor

Peter Cockerill

Publication

Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

Insert

GM-CSF enhancer (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GM-CSF

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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TARDBP

Plasmid

#156073

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TARDBP (TARDBP Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeonGreen

Plasmid

#124101

Purpose

Expression of the fluorescent protein mNeonGreen in mammalian cells

Depositor

Pantelis Tsoulfas

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Mutation

Codon usage was optimized for Homo Sapiens

Promoter

CAG

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCTX-myc-HIPK2 WT

Plasmid

#32996

Depositor

Sergei Sokol

Publication

Hikasa et al Dev Cell. 2010 Oct 19;19(4):521-32.

Insert

HIPK2 (hipk2 Frog)

Tags

6XMyc

Promoter

SCMV

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-DYRK2

Plasmid

#23690

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK2 (DYRK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pgRGFP

Plasmid

#82695

Purpose

Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

Depositor

Alan Mullen

Publication

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

PGK, U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA5 CMV 3xFG eIF4G1 +MIC

Plasmid

#158776

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

Depositor

Benjamin Blencowe

Publication

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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