We narrowed to 13,796 results for: CAN

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pFRT-TODestFLAGHAhFMRPiso1I304N

Plasmid

#48692

Purpose

Expresses hFMRP iso 1 I304N

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 I304N (FMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc-his-BACH1 P47A

Plasmid

#17644

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

To generate the tumor-associated mutant, the prol…

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

Plasmid

#81023

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-479

Promoter

5’LTR

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pCOLADuet-1_nsp10 (SARS-CoV-2)

Plasmid

#169158

Purpose

To express SARS-CoV-2 nsp10 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10 (ORF1ab SARS-CoV-2)

Tags

Methionine

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Ma-sfGFP 150TAG

Plasmid

#197568

Purpose

Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
M. alvus Pyl-tRNA (6) (4x copies)

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PE xCas9

Plasmid

#172652

Purpose

All-in-one prime editor piggyBac transposon (xCas9)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

Plasmid

#154263

Purpose

Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE3G

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

Plasmid

#127867

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse, Human)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

constructed by blunt end fusion of the 5’ 51 nucl…

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-Blasticidin-hOCT4 WT

Plasmid

#198764

Purpose

Mammalian expression of hOCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 WT (POU5F1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PE NG

Plasmid

#174554

Purpose

All-in-one prime editor piggyBac transposon, NG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

Promoter

CAG

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

Plasmid

#169166

Purpose

Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

Insert

3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

Plasmid

#198765

Purpose

expression of hOCT4 mutants

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 236D (POU5F1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S236D

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSico_U6-PGC1a1 sgRNA

Plasmid

#165425

Purpose

Expression of gRNA against human PGC-1a variant 1

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against PGC-1a variant 1 (PPARGC1A Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only
Lenti CGIP + Z-AAT target

Plasmid

#86007

Purpose

lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
Z-AAT target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + TTR target

Plasmid

#86009

Purpose

lenti reporter plasmid with TTR_V30M-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
TTR target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1-S127A/S397A/S94A

Plasmid

#213586

Purpose

Doxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A, S397A, S94A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Proximal Promoter-FLuc2P

Plasmid

#154259

Purpose

Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Proximal Promoter (PPARGC1A Human)

Expression

Mammalian

Promoter

Human PGC-1alpha Proximal Promoter

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Distal Promoter-FLuc2P

Plasmid

#154260

Purpose

Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Distal Promoter (PPARGC1A Human)

Expression

Mammalian

Promoter

Human PGC-1alpha Distal Promoter

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-sfGFP 150TAG

Plasmid

#197570

Purpose

Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
Pyl-tRNA (4 copies)

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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