164,799 results

1. pHAGE-IRES-puro-NLS-HyperdLbCas12a-NLS-3xFlag

   Plasmid

   #236386

   Purpose

   Overexpression of HyperdLbCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   HyperdLbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D156R, D235R, E292R, D350R

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBOBI C-GFP PDZD8

   Plasmid

   #227823

   Purpose

   To amplify the virus that expresses C-GFP PDZD8

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

   Insert

   PDZD8 (PDZD8 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti Puro HA-Ubiquitin-WT

   Plasmid

   #236289

   Purpose

   Lentiviral vector expressing HA-tagged wild type ubiquitin

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available Since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pXJ-His-CD274

   Plasmid

   #232659

   Purpose

   transient expresses PD-L1 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   CD274 (CD274 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Flag-SP1

   Plasmid

   #232649

   Purpose

   expresses SP1 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   SP1 (SP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. act5C-mScarlet

   Plasmid

   #230906

   Purpose

   Ubiquitous expression of mScarlet in Drosophila melanogaster

   Depositor

   Michael Boutros

   Article

   Port et al bioRxiv 2024.11.26.625385

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Coff/Fon-EYFP

   Plasmid

   #231927

   Purpose

   Cre-off/Flp-on EYFP under the Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   eYFP with introns
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p140Tol2-Olactb:SABER

   Plasmid

   #226829

   Purpose

   Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   BFP-SABER
   mCherry-t2A-CreERT2
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Olactb and hsp70

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. JD108

   Plasmid

   #229494

   Purpose

   Adenoviral DeltaF6 plasmid variant devoid of fiber, U exon and pVIII sequences

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   multiple genes
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0381

    Plasmid

    #142256

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMARCA2 (SMARCA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMulti_S_ccdB

    Plasmid

    #223853

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid

    #218973

    Purpose

    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutant SRSF1 binding motif (AGTCTCCT)

    Promoter

    RSV LTR

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid

    #181707

    Purpose

    Galactose inducible expression of Kar2-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Kar2-Beta-Amyloid
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-ORP8-H514A-H515A

    Plasmid

    #208360

    Purpose

    Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    ORP8 (OSBPL8 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    H514A-H515A

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDisplay-GRAB_CRFmut

    Plasmid

    #208656

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-DCX-TurboID-V5

    Plasmid

    #190740

    Purpose

    Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    doublecortin (DCX Human)
    

    Use

    Tags

    TurboID and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-sgKeap1#1/Cre

    Plasmid

    #173639

    Purpose

    Expresses a Keap1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Keap1 (Keap1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX2T-TEV CHMP1B 1-143

    Plasmid

    #108286

    Purpose

    Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-143

    Promoter

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM-TagRFP657

    Plasmid

    #176563

    Purpose

    Expression of TagRFP657 for auxotrophic selection in the absence of methionine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZBTB4-donor

    Plasmid

    #176374

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZBTB4 (ZBTB4 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge