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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,372 results for: BLI

Showing: 5961 - 5980 of 9372 results

  1. SUV420H2

    Plasmid
    #32864
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV420H2 (KMT5C Human)
    Tags
    His tag (with TEV protease)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

    Plasmid
    #105922
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR
    Depositor
    Marcella Patrick
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available Since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105922-plasmid-map-sequence-id-200599

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  4. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51095
    Purpose
    Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    ChIEF(E162A/T198C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E162A/T198C
    Promoter
    EF1a
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51095-plasmid-map-sequence-id-79317

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  5. p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP

    Plasmid
    #185696
    Purpose
    AAV vector expressing cre-dependent mem-mScarlet, T2A, Synaptophysin-EGFP driven by Dlx promoter
    Depositor
    Lynette Lim
    Insert
    mScarlet, T2A, Synaptophysin-EGFP
    Use
    AAV and Cre/Lox
    Tags
    mScarlet palmitoylation, Synaptophysin fused to E…
    Expression
    Mammalian
    Promoter
    mDlx
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185696-plasmid-map-sequence-id-366332

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  6. 4R3H

    Plasmid
    #64652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pDONR221-ACE2

    Plasmid
    #158438
    Purpose
    Gateway Cloning compatible entry vector for the human ACE2 gene.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    ACE2 (ACE2 Human)
    Use
    Gateway entry vector
    Available Since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158438-plasmid-map-sequence-id-308248

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  8. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid
    #72639
    Purpose
    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    Use
    Gateway
    Mutation
    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72639-plasmid-map-sequence-id-429021

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  9. BRDT

    Plasmid
    #38898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRDT (BRDT Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  11. pTXB1-FIS

    Plasmid
    #51564
    Purpose
    Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Francisco Malagon, Sankar Adhya
    Insert
    FIS
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51564-plasmid-map-sequence-id-78815

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  12. FH

    Plasmid
    #38978
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FH (FH Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. EP300

    Plasmid
    #39018
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    EP300 (EP300 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pGEX6P1-DEST-Strep2

    Plasmid
    #119753
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119753-plasmid-map-sequence-id-243311

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  15. pGreenII Tfs-494::LUC

    Plasmid
    #44467
    Purpose
    Expresses a luciferase gene disrupted by an AtCRU3 TALEN Target 494 site and a frame-shift mutation
    Depositor
    Ross Johnson, Roger Hellens
    Insert
    A luciferase gene disrupted by an AtCRU3 TALEN Target 494 site and a frame-shift mutation
    Use
    Luciferase; Plant transformation
    Mutation
    A disruption was made between the first and secon…
    Promoter
    35S Promoter
    Available Since
    April 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    44467-plasmid-map-sequence-id-472941

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  16. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  17. TH mAb_BCN53.1.1D10_HC_Mouse IgG2a

    Plasmid
    #182896
    Purpose
    Mammalian expression of TH mouse mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-TH (human) heavy chain (TH Mouse)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182896-plasmid-map-sequence-id-360557

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  18. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  19. GAK

    Plasmid
    #39042
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GAK (GAK Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid
    #140026
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140026-plasmid-map-sequence-id-275838

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Showing: 5961 - 5980 of 9372 results