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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,358 results for: BLI

Showing: 5961 - 5980 of 8358 results

  1. Hs.HRAS G12D

    Plasmid
    #83183
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12D
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83183-plasmid-map-sequence-id-158146

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  2. pUG-amdSYM-mak10TR

    Plasmid
    #75433
    Purpose
    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae
    Depositor
    John McCusker
    Inserts
    MAK10TR
    ApR
    amdSYM
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminal 740 bp of MAK10 ORF
    Promoter
    Native promoter and PTEF
    Available since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    75433-plasmid-map-sequence-id-142819

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  3. pAG416-GAL-hnRNPA1

    Plasmid
    #181710
    Purpose
    Galactose inducible expression of hnRNPA1
    Depositor
    Alejandro Chavez
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181710-plasmid-map-sequence-id-430953

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  4. pDONR221-CTSB

    Plasmid
    #158443
    Purpose
    Gateway Cloning compatible entry vector for the human CTSB gene.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    CTSB (CTSB Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158443-plasmid-map-sequence-id-308885

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  5. TAF1

    Plasmid
    #39117
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. TAF1

    Plasmid
    #39118
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  8. Hs.NRAS Q61K

    Plasmid
    #83180
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61K
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83180-plasmid-map-sequence-id-158143

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  9. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid
    #78347
    Purpose
    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pLenti-cytoMetGFP

    Plasmid
    #37561
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    only contains cytoplasmic domain amino acids K956…
    Promoter
    CMV
    Available since
    Aug. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    37561-plasmid-map-sequence-id-47323

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  11. dSaCas9-KRAB

    Plasmid
    #205250
    Purpose
    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.
    Depositor
    Rasmus Bak
    Insert
    dSaCas9-KRAB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205250-plasmid-map-sequence-id-409107

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  12. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Promoter
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  14. pLenti-L3US2-RFP-HDAC3

    Plasmid
    #83966
    Purpose
    Lentiviral CRISPR HDAC3 dual gRNA targeting vector
    Depositor
    Eleanor Chen
    Insert
    HDAC3 dual gRNAs (HDAC3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83966-plasmid-map-sequence-id-160487

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  15. tet-pLKO.puro_shHuR CDS

    Plasmid
    #110411
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110411-plasmid-map-sequence-id-213311

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  16. pFRT-TODestFLAGHA_HuB

    Plasmid
    #65755
    Purpose
    expresses FLAGHA-tagged HuB in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL2 (ELAVL2 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    65755-plasmid-map-sequence-id-121638

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  17. XX01CAPN1A-c001

    Plasmid
    #175491
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN1 / CAPNS1 (CAPN1 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175491-plasmid-map-sequence-id-353165

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  18. isoform PKC Iota (JCE597)

    Plasmid
    #182030
    Purpose
    yeast expression of mouse PKC Iota with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Iota (Prkci Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182030-plasmid-map-sequence-id-361960

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  19. pAG416-GAL-TDP-43

    Plasmid
    #181709
    Purpose
    Galactose inducible expression of TDP-43
    Depositor
    Alejandro Chavez
    Insert
    TDP-43 (TARDBP Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181709-plasmid-map-sequence-id-430952

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Showing: 5961 - 5980 of 8358 results