Addgene

We narrowed to 18,510 results for: IRE

Showing: 5961 - 5980 of 18510 results

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p104_gRNA_Sp_CD3e

Plasmid

#213780

Purpose

Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1_SEPT2

Plasmid

#71546

Purpose

mammalian expression of Sept2 with a YFP fusion.

Depositor

Elias Spiliotis, James Nelson

Article

Spiliotis et al Science. 2005 Mar 18;307(5716):1781-5.

Insert

Septin 2 (Septin2 Mouse)

Use

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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C121-E02: ffLuc2

Plasmid

#162889

Purpose

Standard Gateway entry clone encoding firefly luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Olig2

Plasmid

#30131

Depositor

Marius Wernig

Article

Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Zic1

Plasmid

#30132

Depositor

Marius Wernig

Article

Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Insert

Zic1 (Zic1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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SAP145

Plasmid

#11239

Depositor

Robin Reed

Article

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pDB038

Plasmid

#99210

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*), luxAB; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

amber codons at position 29 gIII

Promoter

Ppsp and PproK

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

Plasmid

#135458

Purpose

Mammalian expression of SPARK TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

β2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

Use

Lentiviral

Tags

Flag, V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDB007(+)

Plasmid

#99147

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)

Use

Tags

Expression

Bacterial

Mutation

Promoter

PT7 and PproK

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLexA::Ras

Plasmid

#11340

Depositor

Michael Wigler

Article

Chang et al Cell. 1994 Oct 7. 79(1):131-41.

Insert

Ras1 (ras1 Fission Yeast)

Use

Tags

LexA DNA binding domain

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pBN41

Plasmid

#86716

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Pmyo-2::GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA-POLQ-Halo

Plasmid

#211636

Purpose

sgRNA against POLQ

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA POLQ

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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TtTMPV-Neo

Plasmid

#27993

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TtTMPV-Neo (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-eGFP

Plasmid

#112736

Purpose

Express 2(KW)2-eGFP in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-eGFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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PSICORhp-mp53

Plasmid

#26273

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p53 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
Pdx1 Cre-ER (DM#255)

Plasmid

#15022

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Pdx1 promoter (Pdx1 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre-ER(TM)

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

Plasmid

#216384

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pART (pT-2)

Plasmid

#62708

Purpose

Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA_NEPTUNE_hTLN2-FL

Plasmid

#164842

Purpose

Expression of NEPTUNE tagged FL human talin2

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Use

Tags

His10-NEPTUNE

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

W168Stop; *(see below)

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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