We narrowed to 13,360 results for: Mpl;

1. pT7-V5-SBP-C1-Hs4EBP1_Z

   Plasmid

   #148150

   Purpose

   Mammalian Expression of Hs4EBP1

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   Hs4EBP1 (EIF4EBP1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-T7-hOsa1

   Plasmid

   #17986

   Depositor

   Naoko Tanese

   Article

   Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

   Insert

   Osa1 (ARID1A Human)
   

   Tags

   T7 epitope

   Expression

   Mammalian

   Mutation

   Missing 5' end

   Available Since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

3. TDP-43_CTD_M337P

   Plasmid

   #98675

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337P

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M337P

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TDP-43_CTD_A326P

   Plasmid

   #98672

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with A326P

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A326P

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

   Plasmid

   #199355

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. NSP3C-Flag

   Plasmid

   #210349

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMflET-o4

   Plasmid

   #101314

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   ereB resistance gene
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN mCherry-TAX1BP1(V142S/ΔUBD)

    Plasmid

    #119690

    Purpose

    For mammalian expression of LIR mutant TAX1BP1 without its UBD

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V142S & UBD deletion (1-755), See depositor c…

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWzyE-distrepII

    Plasmid

    #196667

    Purpose

    Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Tags

    TEV-cleavable di-strep tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

    Plasmid

    #186991

    Purpose

    AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
    

    Use

    AAV

    Mutation

    r1045-gggs-r1122 deletion

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199365

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-Neo-gp78/JM20

    Plasmid

    #13303

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78 (AMFR Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBTet-SNAP-CCCAP

    Plasmid

    #136798

    Purpose

    Mammalian expression of the centrosomal protein CCCAP N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CCCAP (SDCCAG8 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.1-nsp7-FLAG

    Plasmid

    #201027

    Purpose

    Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    8xHis and FLAG

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-Med29 pBacPAK8

    Plasmid

    #15369

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    Med29 (MED29 Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159815

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-D-TOPO-kcnn3

    Plasmid

    #164959

    Purpose

    Zebrafish kcnn3 gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnn3 (kcnn3 Zebrafish)
    

    Use

    Pcr cloning vector

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF6 VPS51-V5-6His

    Plasmid

    #175502

    Purpose

    V5 tagged VPS51 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

    Insert

    VPS51 (VPS51 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    A661V- please see depositor comments

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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