We narrowed to 10,783 results for: T7

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pET-6xHis-GFP(+36)

Plasmid

#199165

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt1

Plasmid

#49186

Purpose

cDNA of Akt1 with myristyolation signal in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pIVT-dCas9-EGFP

Plasmid

#174442

Purpose

in vitro transcription template for dCas9-EGFP

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

EGFP

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET11a-hRanGAP1

Plasmid

#50941

Purpose

expression of full length untagged human RanGAP1 in E.coli

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanGTPase activating protein 1 (RANGAP1 Human)

Expression

Bacterial

Promoter

T7

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pTrcHisB-TRF2

Plasmid

#50488

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 3-500

Promoter

pTrc

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET-21(+)Tm9D8* TrpB

Plasmid

#207517

Purpose

Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

Tryptophan synthase β-subunit

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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hDHFR

Plasmid

#184707

Purpose

bacterial expression of human DHFR

Depositor

Peter Wright

Article

Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

Insert

DHFR (DHFR Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-eGFP

Plasmid

#112736

Purpose

Express 2(KW)2-eGFP in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a(+)::NL3F10H

Plasmid

#141290

Purpose

NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC3F10H

Use

Luciferase

Tags

Flag (x3) and His (x10)

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMBP-SpyCatcher

Plasmid

#79053

Purpose

Expresses maltose binding protein (MBP) SpyCatcher fusion

Depositor

James Bowie

Article

Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

Insert

MBP-SpyCatcher

Tags

6xHis and Maltose binding protein

Expression

Bacterial

Mutation

Derived from pDEST14-SpyCatcher (Plasmid #35044)…

Promoter

T7

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET14-GFP15_Encap

Plasmid

#86407

Purpose

Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 15aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCoofy1-psHaloTag1a

Plasmid

#232940

Purpose

Photoswichable HaloTag: psHaloTag1a variant

Depositor

Claire Deo

Article

Walterspiel et al Angew Chem Int Ed Engl. 2025 Oct 23:e202

Insert

photoswitchable HaloTag 1a variant

Tags

His6x-HRV3C

Expression

Bacterial

Mutation

E143W, A258W with linkers L1 (FAG) and L2 (P)

Promoter

T7

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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PET-28a-YTH domain of YTHDF1

Plasmid

#228775

Purpose

Protein expression plasmid for YTH domain of YTHDF1 in E.Coli.

Depositor

Huiqing Zhou

Article

Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

Insert

YTH domain of YTHDF1 (YTHDF1 Human)

Tags

6*His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET23 P53DBD

Plasmid

#174503

Purpose

express P53 DNA binding domain (94-312) in E.coli without tag

Depositor

Stewart Loh

Article

Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

Insert

P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-Venus-StagRFP-AktAR

Plasmid

#173020

Purpose

Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Venus-StagRFP-AktAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-G-L11

Plasmid

#127830

Purpose

Production of recombinant human ribosomal protein L11

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPL11 (RPL11 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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IL-8 toehold switch sensor

Plasmid

#111908

Purpose

Toehold switch sensor to detect IL-8 mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

IL8 toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-NGFP

Plasmid

#52734

Purpose

IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

NGFP+Z-fusion

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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