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We narrowed to 6,132 results for: pCas

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Showing: 5961 - 5980 of 6132 results

  1. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

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  2. sgSf3b1(T1)

    Plasmid
    #90424
    Purpose
    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    mus Sf3b1 gRNA (Sf3b1 Mouse)
    Use
    CRISPR; Guiderna encoding for gene editing
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90424-plasmid-map-sequence-id-190772

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  3. pUDP044

    Plasmid
    #101168
    Purpose
    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101168-plasmid-map-sequence-id-199309

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  5. pCEE

    Plasmid
    #159746
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159746-plasmid-map-sequence-id-313813

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  8. pSA099 PGK1_IVS3-Cas9-BFP

    Plasmid
    #249149
    Purpose
    Overexpression of SpCas9-BFP with PGK1 IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PGK1_IVS3-Cas9-TagBFP (PGK1 S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    PGK1 IVS3 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249149-plasmid-map-sequence-id-496391

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  9. pSA100 RPS12_IVS1-Cas9-BFP

    Plasmid
    #249150
    Purpose
    Overexpression of SpCas9-BFP with RPS12 IVS1 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    RPS12_IVS1-Cas9-TagBFP (RPS12 S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    RPS12 IVS1 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249150-plasmid-map-sequence-id-496392

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  10. pSA093 PFDN2_IVS3-Cas9-BFP

    Plasmid
    #249143
    Purpose
    Overexpression of SpCas9-BFP with PFDN2 IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PFDN2_IVS3-Cas9-TagBFP (PFDN2 S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    PFDN2 IVS3 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249143-plasmid-map-sequence-id-496387

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  11. pSA098 RPL18A_IVS4-Cas9-BFP

    Plasmid
    #249148
    Purpose
    Overexpression of SpCas9-BFP with RPL18A IVS4 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    RPL18A_IVS4-Cas9-TagBFP (RPL18A S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    RPL18A IVS4 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249148-plasmid-map-sequence-id-496386

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  16. pSCBE3-HF1

    Plasmid
    #218156
    Purpose
    This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218156-plasmid-map-sequence-id-434901

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  17. pSCBE3-HF1-Hypa

    Plasmid
    #218158
    Purpose
    This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218158-plasmid-map-sequence-id-433272

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  18. pSCBE3-Hypa

    Plasmid
    #218157
    Purpose
    This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N692A…
    Promoter
    rpsL promoter
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    218157-plasmid-map-sequence-id-433172

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  19. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid
    #208966
    Purpose
    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…
    Promoter
    CMV and T7
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208966-plasmid-map-sequence-id-422883

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  20. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid
    #208973
    Purpose
    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208973-plasmid-map-sequence-id-415830

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Showing: 5961 - 5980 of 6132 results