We narrowed to 10,611 results for: t7

1. pET303-colE1TR

   Plasmid

   #155181

   Purpose

   Expresses colicin E1 residues 1-364

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-364
   

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-364

   Promoter

   T7

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLP-pTac-Tn5-chlorR

   Plasmid

   #199994

   Purpose

   Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Landing Pads
   

   Expression

   Bacterial

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fast-GCaMP-EF20

   Plasmid

   #52645

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF20
   

   Expression

   Mammalian

   Mutation

   D362N/D366N mutated from GCaMP3

   Promoter

   T7

   Available Since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pTK312

   Plasmid

   #59569

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP XbaI attB1 Kozak EGFP ochre attB2 AscI
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETb-ffDronpa

   Plasmid

   #78182

   Purpose

   Expresses ffDronpa E. coli strains

   Depositor

   Peter Dedecker

   Article

   Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

   Insert

   ffDronpa
   

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUMO-YHRC

   Plasmid

   #54336

   Purpose

   Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

   Depositor

   Claes Andréasson

   Article

   Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

   Type

   Empty backbone

   Tags

   6His-SUMO (yeast gene: SMT3)

   Expression

   Bacterial

   Promoter

   T7-LacO

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_Caspase-5-enzymatic-domain

   Plasmid

   #214306

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP5 (CASP5 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   aa133-434

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TTR C10S S85C

   Plasmid

   #184410

   Purpose

   bacterial expression of TTR

   Depositor

   Peter Wright

   Article

   Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   C10S, S85C

   Promoter

   T7

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMCSGC-GB1

   Plasmid

   #186786

   Purpose

   E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

   Depositor

   Alexei Savchenko

   Article

   Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2430

    Plasmid

    #36829

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HDAC2-TALEN_Left (Hdac2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. RhGC(BE)_pGEM

    Plasmid

    #85469

    Purpose

    The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

    Depositor

    Peter Hegemann

    Article

    Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

    Insert

    rhodopsin-guanylyl cyclase
    

    Expression

    Mammalian

    Promoter

    T7 Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-B4E

    Plasmid

    #162067

    Purpose

    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E

    Depositor

    Karen Polizzi

    Article

    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1

    Insert

    ampR-eIF4E fusion protein
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    eIF4E incorporating the K119A mutation, which is …

    Promoter

    T7

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. saposin B^W

    Plasmid

    #161759

    Purpose

    Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

    Depositor

    Sam Gellman

    Article

    Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

    Insert

    saposin B R38_W
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    changed Arginine 38 to Tryptophan

    Promoter

    T7

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-AktAR-Kras

    Plasmid

    #123357

    Purpose

    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-M1-S1-S2-S4T1L

    Plasmid

    #33306

    Depositor

    Terence Dermody

    Article

    Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

    Inserts

    S2 (MoV1sS2gp1 Orthoreovirus T1L)
    S1 (MoV1sS1gp2, MoV1sS1gp1 Orthoreovirus T1L)
    S4 (MoV1sS4gp1 Orthoreovirus T1L)
    M1 (M1 Orthoreovirus T1L)
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. Twitch-1 pRSETB

    Plasmid

    #48207

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Mues et al Nat Med. 2013 Jun;19(6):778-83. doi: 10.

    Insert

    Twitch-1
    

    Tags

    6xHIS, ECFP, and cpCit174

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pMC051-HisMS2_PLP_pac_LC

    Plasmid

    #128234

    Purpose

    Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

    Depositor

    Paul Freemont

    Article

    de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

    Inserts

    Maturation Protein
    Coat Protein Dimer
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET51b-NAD-cpLuc2

    Plasmid

    #135629

    Purpose

    Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

    Depositor

    Kai Johnsson

    Article

    Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

    Insert

    NAD-cpLuc2 sensor
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21b-LaG9(S151C)

    Plasmid

    #172490

    Purpose

    Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    LaG9(S151C)
    

    Tags

    6xHis, PPX cleavage site, and pelB leader sequenc…

    Expression

    Bacterial

    Mutation

    S151C

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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