-
Plasmid#128377PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the HYG resistance marker.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only
-
pPEE31
Plasmid#128371PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the nourseothricin resistance marker (NAT)DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEE39
Plasmid#128379PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the HYG resistance marker.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEE33
Plasmid#128373PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the NEO resistance markerDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEE29
Plasmid#128369PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the nourseothricin resistance marker (NAT)DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEE28
Plasmid#128368PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the nourseothricin resistance marker (NAT)DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEE34
Plasmid#128374PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the NEO resistance markerDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEE30
Plasmid#128370PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the nourseothricin resistance marker (NAT)DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSDMA57
Plasmid#67941PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains NEO resistance marker.DepositorTypeEmpty backboneUseCryptococcal complementation vectorTagsExpressionMutationPromoterACT1 for Cryptococcal markerAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSDMA58
Plasmid#67942PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains HYG resistance marker.DepositorTypeEmpty backboneUseCryptococcal complementation vectorTagsExpressionMutationPromoterACT1 for Cryptococcal markerAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRKO
Plasmid#49456PurposeloxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptionsDepositorInsertloxP-kanMX-loxP cassette
UseCre/LoxTagsExpressionBacterialMutationPromoternoneAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSDMA25
Plasmid#67940PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains nourseothricin resistance marker (NAT)DepositorTypeEmpty backboneUseCryptococcal complementation vectorTagsExpressionMutationPromoterACT1 for Cryptococcal markerAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoteract1Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG705_lexO-AsiSI_LexA-TF_LEU2MX
Plasmid#154817PurposeInducible expression of AsiSI endonuclease in S. cerevisiaeDepositorInsertsAsiSI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG706_lexO-SbfI_LexA-TF_LEU2MX
Plasmid#154818PurposeInducible expression of SbfI endonuclease in S. cerevisiaeDepositorInsertsSbfI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG659_lexO-FseI_LexA-TF_LEU2MX
Plasmid#154816PurposeInducible expression of FseI endonuclease in S. cerevisiaeDepositorInsertsFseI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG713_lexO-HO_LexA-TF_URA3MX
Plasmid#154821PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsUseTagsFLAGExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0661
Plasmid#133476PurposeExpression in fission yeast from promoter pact1, integrated at ade6 locusDepositorInsertpAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRG714_lexO-AscI_LexA-TF_URA3MX
Plasmid#154822PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG646_lexO-HO_LexA-TF_LEU2MX
Plasmid#154815PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsUseTagsFLAGExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG645_lexO-AscI_LexA-TF_LEU2MX
Plasmid#154813PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0328
Plasmid#133489PurposeExpression of nucleo-cytoplasmic mCherryDepositorInsertpUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSDMA65
Plasmid#128354PurposeCodon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterACT1Available sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRG710_lexO-HO_LexA-TF_HIS3MX
Plasmid#154819PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsUseTagsFLAGExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0327
Plasmid#133488PurposeExpression of nucleo-cytoplasmic sfGFPDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0783
Plasmid#133515PurposeExpression of mCherry tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0663
Plasmid#133478PurposeExpression in fission yeast from promoter pact1, integrated at his5 locusDepositorInsertpHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0714
Plasmid#133479PurposeExpression in fission yeast from promoter pact1, integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0771
Plasmid#133513PurposeExpression of mTagBFP2 tagged CHD domain to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only