We narrowed to 2,515 results for: GCG

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pJAM1.21_PB-UGCG-PGK-Blast

Plasmid

#205566

Purpose

Express human UGCG

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

UGCG (UGCG Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GCGR-NTEV-TCS-GV-2xHA

Plasmid

#194374

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GCGR-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F3bbs kan NNNTGGGCGk

Plasmid

#18756

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F1bbs kan GCGTGGNNN

Plasmid

#18753

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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sHiBiT-GCGR Fusion Vector

Plasmid

#236837

Purpose

Express GCGR in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

sHiBiT-GCGR (GCGR Human)

Tags

HiBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

Plasmid

#218860

Purpose

10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCG-ICAM(1235)-nrxn1b

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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elavl3:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218852

Purpose

D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218857

Purpose

Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHA

Plasmid

#194375

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194376

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194377

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

Plasmid

#121175

Purpose

Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

Depositor

Timo Otonkoski

Insert

GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro

Use

CRISPR

Expression

Mammalian

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A,K276A, R280A,K284A]

Plasmid

#188599

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A,K276A]

Plasmid

#188596

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG], K276A [AAG=>GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAWp92

Plasmid

#157975

Purpose

pBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone32 (Opti-SpCas9)

Plasmid

#131736

Purpose

Expresses Opti-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

Opti-SpCas9

Use

Lentiviral

Tags

Flag

Mutation

R661A+K1003H

Promoter

EFS

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp100

Plasmid

#157977

Purpose

pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp40

Plasmid

#157980

Purpose

pFUGW-EFSp-BFP-{SbfI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone3-12 (OptiHF-SpCas9)

Plasmid

#131737

Purpose

Expresses OptiHF-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

OptiHF-SpCas9

Use

Lentiviral

Tags

Flag

Mutation

Q695A+K848A+E923M+T924V+Q926A

Promoter

EFS

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp28-v1 scaffold

Plasmid

#157976

Purpose

pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAWp102-v2 scaffold

Plasmid

#157978

Purpose

pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3746

Plasmid

#154109

Purpose

loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*

Plasmid

#32389

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st E2F binding site mutation: GCG-->AAA

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pSUPER-scramble

Plasmid

#118349

Purpose

This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

scrambled shRNA

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pNAS1B

Plasmid

#61968

Purpose

Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

mCherry
GFP

Expression

Bacterial

Mutation

Serine 65 to Glycine (GGT), Serine 72 to Alanine …

Promoter

PBAD and PLtetO

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-shCtnnd2.2-GFP

Plasmid

#209097

Purpose

GFP expressing shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.2

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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MYMH 47

Plasmid

#138038

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-8AC-GFP

Expression

Bacterial

Mutation

AAA GAG GCG AAA

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 29

Plasmid

#138029

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-5AC-GFP

Expression

Bacterial

Mutation

AAA GCG GAG AAA

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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