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Plasmid#25539PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPAPSS2 (PAPSS2 Human)
UseTagsHisExpressionBacterialMutationContains kinase domain (residues 21-218)PromoterAvailable sinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOC4 Kcnn2-smFP-myc KI
Plasmid#183438PurposeFlpON knock-in for SK2-smFP-myc (amino acid position: T571)DepositorInsertgRNA and smFP myc donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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MmSphk2
Plasmid#118598PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PAPSS2 gRNA (BRDN0001145342)
Plasmid#76316Purpose3rd generation lentiviral gRNA plasmid targeting human PAPSS2DepositorInsertPAPSS2 (PAPSS2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAPSS2 gRNA (BRDN0001147912)
Plasmid#76317Purpose3rd generation lentiviral gRNA plasmid targeting human PAPSS2DepositorInsertPAPSS2 (PAPSS2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAPSS2 gRNA (BRDN0001146774)
Plasmid#76318Purpose3rd generation lentiviral gRNA plasmid targeting human PAPSS2DepositorInsertPAPSS2 (PAPSS2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E293 Hs.RPS6KA3
Plasmid#70577PurposeGateway ORF clone of human RPS6KA3 [NM_004586.2] with stop codon (for native or N-terminal fusions)DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E294 Hs.RPS6KA3-nostop
Plasmid#70578PurposeGateway ORF clone of human RPS6KA3 [NM_004586.2] without stop codon (for C-terminal fusions)DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
CB6-GFP-TAOK1
Plasmid#197108PurposeExpression of GFP-tagged TAOK1 / PSK2 in mammalian cellsDepositorInsertTAOK1 (TAOK1 Human)
UseTagsGFPExpressionMammalianMutationWild TypePromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CB6-GFP-TAOK1 (K57A)
Plasmid#197109PurposeExpression of kinase-deficient GFP-tagged TAOK1 (K57A) / PSK2 (K57A) in mammalian cellsDepositorInsertTAOK1 (K57A) (TAOK1 Human)
UseTagsGFPExpressionMammalianMutationChanged K 57 to APromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA3 gRNA (BRDN0001144943)
Plasmid#75942Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KA3DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA3 gRNA (BRDN0001148452)
Plasmid#75943Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KA3DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA3 gRNA (BRDN0001147852)
Plasmid#75944Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KA3DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag RPSK6A3
Plasmid#20627DepositorInsertRPSK6A3 (RPS6KA3 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-RPS6KA3
Plasmid#23527DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
TIGRE docking site targeting vector
Plasmid#61580PurposeCreate a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchangeDepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRMCD28
Plasmid#167556PurposePlasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsPhoA'ExpressionBacterialMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAP3K6-TAP
Plasmid#69727PurposeExpress tandem tagged MAP3K6 in cellsDepositorInsertApoptosis signal-regulating kinase 2 (MAP3K6 Human)
UseTagsHA-V5 tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRMCD70
Plasmid#167557PurposePlasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsLacZ'ExpressionBacterialMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12D
Plasmid#58902PurposeExpresses human Kras with a G12D MutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12D mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12C
Plasmid#58901PurposeExpresses human Kras with a G12C mutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12C mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras S17N
Plasmid#58899PurposeExpresses human Kras with a S17N mutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationS17N mutationPromoterAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G15A
Plasmid#58898PurposeExpresses human Kras with a G15A mutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG15A mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12V-T35S
Plasmid#58900PurposeExpresses human Kras with G12V and T35S mutationsDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12V and T35S mutations (effector binding mutant)PromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rasgrf2-2A-dCre targeting vector
Plasmid#61573PurposeTarget a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 geneDepositorInsertRasgrf2-2A-dCre (Rasgrf2 Mouse, P1 bacteriophage)
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pvalb-2A-Cre targeting vector
Plasmid#61570PurposeTarget the Cre recombinase gene to the stop codon of the mouse Pvalb geneDepositorInsertPvalb exon 4 - 2A - Cre
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Snap25-LSL-2A-GFP targeting vector
Plasmid#61575PurposeTarget a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 geneDepositorInsertSnap25-LSL-2A-GFP
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS3 (CK2alpha, CK2beta)
Plasmid#27092DepositorUseTetracycline-regulated expressionTagsHA and MycExpressionMammalianMutationPromoterbidirectional tet-responsive promoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PAPSS2
Plasmid#23581DepositorInsertPAPSS2 (PAPSS2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only