Addgene

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pSTARR-seq_fly-NipB

Plasmid

#71502

Purpose

flySTARR-seq screening vector with NipB core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar1

Plasmid

#232738

Purpose

Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p413 TEF NAPstar2

Plasmid

#232739

Purpose

Expresses NADPH/NADP+ biosensor NAPstar2 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar4

Plasmid

#232741

Purpose

Expresses NADPH/NADP+ biosensor NAPstar4 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar6

Plasmid

#232742

Purpose

Expresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar7

Plasmid

#232744

Purpose

Expresses NADPH/NADP+ biosensor NAPstar7 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar7

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pC016-gPsp-EGFP_start_codon

Plasmid

#233613

Purpose

Expression of guide RNA targeting the start codon of the EGFP gene for PspCas13b

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

gRNA targeting EGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Stargazin-EBFP2-FRB

Plasmid

#222417

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

Cacng2 (Cacng2 Mouse)

Use

Tags

EBFP2-HA-FRB

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 2

Plasmid

#99317

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-C

Plasmid

#87540

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-G

Plasmid

#87541

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALE-STAR-KRAB-T

Plasmid

#87538

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALE-STAR-KRAB-A

Plasmid

#87539

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-x16

Plasmid

#71505

Purpose

flySTARR-seq screening vector with x16 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-eEF1delta

Plasmid

#71501

Purpose

flySTARR-seq screening vector with eEF1delta core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTagBFP2-blast

Plasmid

#125239

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTagBFP2 (codon optimized)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Plasmid

#222991

Purpose

Bacterial expression of iGeoCas9(C2) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-Stargazin-mEGFP-iLID

Plasmid

#178529

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL4-3 envFS eGFP NoStarts

Plasmid

#101342

Purpose

All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

No methonines in Gag

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-TARPGamma2/Stargazin [N245/1R]

Plasmid

#177521

Purpose

Mammalian Expression Plasmid of anti-TARPGamma2/Stargazin (Rat). Derived from hybridoma N245/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TARPGamma2/Stargazin (Rattus norvegicus) recombinant mouse monoclonal antibody (Cacng2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2186

Plasmid

#40386

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Expression

Mutation

WT aa1867-2186

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus_Sumostar/LRRK2 1867-2161_T2031E

Plasmid

#40395

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Expression

Mutation

aa1867-2161, T2031E

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEGFP-Map7-Start-1351

Plasmid

#46075

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-Start-1351 (Map7 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBACgus_Sumostar/LRRK2 1867-2186_T2031E

Plasmid

#40389

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Expression

Mutation

aa1867-2186, T2031E

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
hSTARR-seq_OCT4 3UTR vector

Plasmid

#99295

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBACgus_Sumostar/LRRK2 1867-2186_G2019S

Plasmid

#40387

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Expression

Mutation

aa1867-2186, G2019S

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus_Sumostar/LRRK2 1867-2176

Plasmid

#40392

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Expression

Mutation

aa1867-2176

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus_Sumostar/LRRK2 1867-2161

Plasmid

#40391

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Expression

Mutation

aa1867-2161

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)

Plasmid

#159889

Purpose

Three-fragment Gateway compatible promoter for expression in all muscles of C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

[4-1] - Promoter - Pmlc-2(no_start)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L4R1-hCMVstar1+bgloInt

Plasmid

#139528

Purpose

Provides conditional promoter cassette between attL4-attR1 on Gateway entry vector

Depositor

Minoru Ko

Insert

hCMVstar1 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALE-STAR-T_RFP entry

Plasmid

#87526

Purpose

Destination vector for domain insertion

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale empty functiona…

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALE-STAR-A_RFP entry

Plasmid

#87527

Purpose

Destination vector for domain insertion

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale empty functiona…

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALE-STAR-C_RFP entry

Plasmid

#87528

Purpose

Destination vector for domain insertion

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale empty functiona…

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALE-STAR-G_RFP entry

Plasmid

#87529

Purpose

Destination vector for domain insertion

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale empty functiona…

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBACgus_Sumostar/LRRK2 1867-2141

Plasmid

#40390

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Expression

Mutation

aa1867-2141

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus_Sumostar/LRRK2 1867-2161_G2019S

Plasmid

#40393

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Expression

Mutation

aa1867-2161, G2019S

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
STARR-seq luciferase validation vector_ORI_empty

Plasmid

#99297

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
STARR-seq luciferase validation vector_ORI_neg.cont

Plasmid

#99315

Purpose

Negative control for luciferase validation vectors

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

neutral region; chr3: 55198740-55200041

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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