1. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

   Plasmid

   #107726

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

   Plasmid

   #107727

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX458-sgRNA_Ago1_1

   Plasmid

   #73533

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX458-sgRNA_Ago1_2

   Plasmid

   #73534

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

5. PX458_KMT2B_2

   Plasmid

   #101075

   Purpose

   Encodes gRNA for 3' target of human KMT2B

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KMT2B (KMT2B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PX458_KMT2B_1

   Plasmid

   #101074

   Purpose

   Encodes gRNA for 3' target of human KMT2B

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KMT2B (KMT2B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX458-sgRNA_Ago2_3

   Plasmid

   #73531

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX458-sgRNA_Ago2_4

   Plasmid

   #73532

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

   Plasmid

   #107728

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JARID2-KO-gRNA-1

    Plasmid

    #131328

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

11. JARID2-KO-gRNA-2

    Plasmid

    #131327

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

12. pX458-sgRNA_Ago1_3

    Plasmid

    #73535

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

13. PX458_RERE_1

    Plasmid

    #101073

    Purpose

    Encodes gRNA for 3' target of human RERE

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against RERE (RERE Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

    Plasmid

    #197424

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

    Plasmid

    #197425

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

    Plasmid

    #197426

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Y365A or A365Y inducible rescue

    Plasmid

    #131323

    Purpose

    gRNA to generate i-WT-r or i-MT-r systems

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Y365A or A365Y inducible rescue
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MTF2_KO_ex11_gRNA-4

    Plasmid

    #131334

    Purpose

    gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    MTF2_KO_ex11_gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LMX1B-gRNA-C-del

    Plasmid

    #131516

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

20. LMX1B-gRNA-N-del

    Plasmid

    #131333

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-N-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge