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SepOTSε

Plasmid

#68286

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSθ

Plasmid

#68289

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0079

Plasmid

#117646

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0016

Plasmid

#117526

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.0

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.0 (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

K810A, K1003A, R1060A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSν

Plasmid

#68294

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 5xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSβ

Plasmid

#68283

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 1xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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insulator_seq_reporter_vector

Plasmid

#221460

Purpose

Vector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

gypsy insulator-EGFP-neutral spacer DNA-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_ZF1_mNeonGreen

Plasmid

#213834

Purpose

Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen_ZF2

Plasmid

#213835

Purpose

Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_ZF2_mNeonGreen

Plasmid

#213836

Purpose

Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen_ZF1

Plasmid

#213837

Purpose

Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_ZF1_mNeonGreen

Plasmid

#213840

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen

Plasmid

#213841

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_TetR

Plasmid

#213847

Purpose

Facilitates genomic integration of tetR expression cassette.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

TetR

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMX_mePOU

Plasmid

#206396

Purpose

Expresses mouse ePOU in mammalian cells, for retrovirus generation.

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM_mePOU_3xFlag

Plasmid

#206395

Purpose

Expresses 3xflag fused mouse ePOU in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEPOZ0CM0138

Plasmid

#197533

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

ARF18_w/o_stop

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge