Skip to main content
Addgene
Showing: 41 - 60 of 539 results

  13. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

    Enlarge

  17. pBN095

    Plasmid
    #173765
    Purpose
    express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    delete motif 3
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173765-plasmid-map-sequence-id-344806

    Enlarge

  18. pBN215

    Plasmid
    #167962
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167962-plasmid-map-sequence-id-344789

    Enlarge

  19. pBN216

    Plasmid
    #167963
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167963-plasmid-map-sequence-id-344805

    Enlarge

  20. pBN217

    Plasmid
    #167964
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167964-plasmid-map-sequence-id-344780

    Enlarge

Showing: 41 - 60 of 539 results