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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 145 results for: acr Anti-CRISPR

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Showing: 41 - 60 of 145 results

  1. pAcrF2

    Plasmid
    #89234
    Purpose
    Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Publication
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcrF2
    Tags
    6xHis-TEV
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89234-plasmid-map-sequence-id-177169

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  2. pVAX_CBI_AcrIIA2

    Plasmid
    #136661
    Purpose
    Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites
    Depositor
    Yannick Doyon
    Publication
    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcrIIA2
    Use
    Synthetic Biology
    Tags
    No tag, No NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    136661-plasmid-map-sequence-id-273234

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  3. pAcrF1

    Plasmid
    #89233
    Purpose
    Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Publication
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcrF1
    Tags
    6xHis-TEV and FLAG
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89233-plasmid-map-sequence-id-177171

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  5. AcrIIC1X

    Plasmid
    #128112
    Purpose
    AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9
    Depositor
    Dominik Niopek
    Publication
    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcrX (AcrIIC1 N3F/D15Q/A48I)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128112-plasmid-map-sequence-id-249186

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  12. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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Showing: 41 - 60 of 145 results