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Showing: 41 - 60 of 188 results

  9. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

    Plasmid
    #233039
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233039-plasmid-map-sequence-id-463773

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  11. pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233033
    Purpose
    To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    DjCas13d and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233033-plasmid-map-sequence-id-464626

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  13. pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233034
    Purpose
    To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233034-plasmid-map-sequence-id-463768

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  14. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

    Plasmid
    #228360
    Purpose
    hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36
    Depositor
    Michael Boettcher
    Article
    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral; Rfxcas13d grna expression …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    228360-plasmid-map-sequence-id-454335

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  16. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid
    #138150
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    DR1 A>T
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138150-plasmid-map-sequence-id-350242

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  17. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  18. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  19. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138148-plasmid-map-sequence-id-318593

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Showing: 41 - 60 of 188 results