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pEGFP-C1 beta-catenin Y654F

Plasmid

#16072

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Y654F, *(see below)

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 beta-catenin Y654E

Plasmid

#16073

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Y654E, *(see below)

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-SpyCatcher3

Plasmid

#235149

Purpose

Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

SpyCatcher3

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmcherry α-catenin L344P

Plasmid

#178647

Purpose

express α-catenin with L344P mutation

Depositor

Daniel Conway

Article

Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

Insert

α-catenin (CTNNA1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

L344P

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-human beta-catenin full length

Plasmid

#132609

Purpose

expresses human beta-catenin full length in E. coli

Depositor

Wenqing Xu

Article

Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

Insert

beta-catenin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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14-3-3 KO vector

Plasmid

#16551

Depositor

Bert Vogelstein

Article

Chan et al Nature. 1999 Oct 7. 401(6753):616-20.

Insert

14-3-3 knockout (SFN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 psi

Plasmid

#44931

Depositor

Robert Ferl

Article

Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

Insert

14-3-3 psi (GRF3 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 omega

Plasmid

#44930

Depositor

Robert Ferl

Article

Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

Insert

14-3-3 omega (GRF2 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 upsilon

Plasmid

#44932

Depositor

Robert Ferl

Article

Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

Insert

14-3-3 upsilon (GRF5 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
HA-14-3-3 eta

Plasmid

#116887

Purpose

Expresses HA-tagged human 14-3-3 eta in mammalian cells

Depositor

Ken-Ichi Takemaru, FENG-QIAN Li

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 eta (YWHAH Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 zeta

Plasmid

#116888

Purpose

Expresses HA-tagged human 14-3-3 zeta in mammalian cells

Depositor

Ken-Ichi Takemaru, FENG-QIAN Li

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 zeta (YWHAZ Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-14-3-3 epsilon

Plasmid

#116886

Purpose

Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

Depositor

Ken-Ichi Takemaru, FENG-QIAN Li

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 epsilon (YWHAE Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBS 14-3-3 zeta

Plasmid

#19226

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Tyr3/Trp5 monooxygenase (YWHAZ Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
V5-14-3-3-e

Plasmid

#229426

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

YWHAE (YWHAE Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

Plasmid

#200636

Purpose

Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmcherry α-catenin ΔVBS

Plasmid

#178649

Purpose

express α-catenin without a vinculin binding site (VBS)

Depositor

Daniel Conway

Article

Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

Insert

α-catenin (CTNNA1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

VBS of alpha catenin was removed and replaced wit…

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pVP1/3

Plasmid

#73548

Purpose

Expresses VP1 and VP3 for Adeno-associated virus serotype 2

Depositor

Junghae Suh

Article

Gomez et al ACS Nano. 2015 Nov 30.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCA_e-3

Plasmid

#202098

Purpose

Receiver cloning vector for even-3 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_o-3

Plasmid

#202093

Purpose

Receiver cloning vector for odd-3 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pTUB1:OsTIR1-3FLAG, SAG1:CAT

Plasmid

#87258

Purpose

TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

Depositor

David Sibley

Article

Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

Inserts

TIR1
CAT

Use

Toxoplasma expression

Tags

3FLAG

Expression

Mutation

Codon optimized for Toxoplasma gondii expression

Promoter

TgSAG1 361 bp and TgTUB1 2717 bp

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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