-
Plasmid#222587PurposeBB3 gsdA tet-on overexpression cassette with homologous flanks for integration at pyrG locusDepositorArticleInsertgsdA (gsdA )
UseA. nigerTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insert,…Promotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF8
Plasmid#222592PurposeBB2 gsdA tet-on overexpression cassetteDepositorArticleInsertgsdA
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insert,…Promotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-RR-zrif1_exon8
Plasmid#194434PurposeRight (RR) TALEN for mutating zebrafish rif1 geneDepositorInsertzrif1_ex8_RIGHT (rif1 Zebrafish)
UseTALENTagsExpressionMutationPromoterCMV IE94Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-DD-zrif1_exon8
Plasmid#194435PurposeLeft (DD) TALEN for mutating zebrafish rif1 geneDepositorInsertzrif1_ex8_LEFT (rif1 Zebrafish)
UseTALENTagsExpressionMutationPromoterCMV IE94Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GATA1
Plasmid#194436PurposeIn Situ hybridization probesDepositorInsertGATA1a (gata1a Zebrafish)
UseIn situ hybridizationTagsExpressionMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTT]-nLuc
Plasmid#199757PurposeElongation reporter construct to quantify elongation of synYFP[CTT]DepositorInsertleuYFP[CTT]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTC]-nLuc
Plasmid#199758PurposeElongation reporter construct to quantify elongation of synYFP[CTC]DepositorInsertleuYFP[CTC]
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTW072
Plasmid#190247PurposeT-DNA for arabidopsis transformationDepositorInsertgRNA, Cas9, firefly luciferase, bar
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW069
Plasmid#190246PurposeT-DNA for arabidopsis transformationDepositorInsertgRNA, Cas9, firefly luciferase, bar
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzpHS2
Plasmid#172971PurposepWSK29 with S. flexneri wzzpHS2 geneDepositorInsertwzzPHS2 gene from Shigella flexneri
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzO139
Plasmid#172970PurposepWSK29 with Vibrio cholerae wzzO139 geneDepositorInsertwzz gene from Vibrio cholerae O139
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBSF
Plasmid#172967PurposepWSK29 with S. flexneri 2a wzzBSF geneDepositorInsertwzzB gene from Shigella flexneri
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA E171Q HDEL
Plasmid#32123DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationK108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA K108A HDEL
Plasmid#32121DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationK108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM197
Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-GGS4X-GFP
Plasmid#108583PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
PKAcs-GGSD5X-GFP
Plasmid#108584PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE GFP
Plasmid#32120DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303PromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only