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Showing: 41 - 60 of 10489 results

  2. pcDNA3.1(+)-Mito-CaNARi

    Plasmid
    #64851
    Purpose
    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CaNARi
    Use
    Tags
    Cerulean, N-terminal 30 amino acids from DAKAP1, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64851-plasmid-map-sequence-id-109676

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  3. pLNHA-C1-HsNot1iso1-shRNAres_V

    Plasmid
    #147749
    Purpose
    Mammalian Expression of HsNot1iso1-shRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147749-plasmid-map-sequence-id-444359

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  8. pUt-MONARCH 1.0_crEGFP

    Plasmid
    #224790
    Purpose
    LPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selection
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    crEGFP
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224790-plasmid-map-sequence-id-450828

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  13. pEYFP-N1-HsSmg5-siRNAres_I

    Plasmid
    #146541
    Purpose
    Mammalian Expression of HsSmg5-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    three non silent mutation A254G (K85R), T980C (L3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146541-plasmid-map-sequence-id-288987

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  14. pAc5.1B-HA-DmMe31B-dsRNAres_I

    Plasmid
    #146528
    Purpose
    Insect Expression of DmMe31B-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmMe31B-dsRNAres (me31B Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation A1101G compared to the sequen…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146528-plasmid-map-sequence-id-288974

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  16. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid
    #182887
    Purpose
    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    silet mutations in aa 197 - 203 to confer resista…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182887-plasmid-map-sequence-id-367932

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  17. pcDNA-rUSP19ER-siRNAresistant

    Plasmid
    #155251
    Purpose
    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155251-plasmid-map-sequence-id-313953

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  18. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid
    #155252
    Purpose
    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155252-plasmid-map-sequence-id-313955

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Showing: 41 - 60 of 10489 results