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pCW57.1

Plasmid

#41393

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7

Plasmid

#136466

Purpose

Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-M2

Plasmid

#44170

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Influenza A virus M2 matrix gene

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_full-length_bridge_RNA_polIII_term

Plasmid

#251495

Purpose

Expression of the native full length ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge RNA

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_TBL_bridge_RNA_polIII_term

Plasmid

#251496

Purpose

Expression of the Target Binding Loop (TBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge RNA Target Binding Loop (TBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_DBL_bridge_RNA_polIII_term

Plasmid

#251497

Purpose

Expression of the Donor Binding Loop (DBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge RNA Donor Binding Loop (DBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pMXs-3XMyc-EGFP-OMP25

Plasmid

#83355

Purpose

For tagging mitochondria with Myc epitopes (Control-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XMyc-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-M2rtTA

Plasmid

#20342

Purpose

Lentiviral plasmid expressing the reverse tetracycline transactivator

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

M2rtTA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pInducer20

Plasmid

#44012

Purpose

Tet-inducible lentiviral vector for ORF expression

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#162606

Purpose

Pre-express Cas9 in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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