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We narrowed to 8,771 results for: ef1

Showing: 291 - 300 of 8771 results

  2. pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

    Plasmid
    #162804
    Purpose
    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080
    Insert
    Ub-BsmBI_IRES_Blasticidin
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    162804-plasmid-map-sequence-id-318670

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  3. PB_TRE_AP4-PDGFR_EF1a_eBFP2

    Plasmid
    #186306
    Purpose
    Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    AP4-PDGFR
    eBFP2
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186306-plasmid-map-sequence-id-366944

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  4. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

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  8. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

    Plasmid
    #194461
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ΞΆ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-JAG1 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194461-plasmid-map-sequence-id-384277

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Showing: 291 - 300 of 8771 results