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pSKAP_AA

Plasmid

#122703

Purpose

pSKAP plasmid containing the unique DNA barcode "AA" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AA

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AO

Plasmid

#122717

Purpose

pSKAP plasmid containing the unique DNA barcode "AO" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AO

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AI

Plasmid

#122711

Purpose

pSKAP plasmid containing the unique DNA barcode "AI" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AI

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AH

Plasmid

#122710

Purpose

pSKAP plasmid containing the unique DNA barcode "AH" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AH

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AG

Plasmid

#122709

Purpose

pSKAP plasmid containing the unique DNA barcode "AG" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AG

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AE

Plasmid

#122707

Purpose

pSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AE

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP II (pMIG II)

Plasmid

#52107

Purpose

a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pGTy1mhis3-AI or pGTy1his3-AI

Plasmid

#62228

Purpose

A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

Depositor

David Garfinkel

Article

Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

Insert

Ty1H3 retrotransposon

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CAG-rtTA

Plasmid

#185562

Purpose

Ubiquitous expression of reverse tetracycline transactivator for TetON system

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

rtTA

Expression

Mammalian

Promoter

CAG

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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XE240 Fz1-ER-GFP

Plasmid

#16815

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

Fz1-ER-GFP

Use

Xenopus expression

Tags

gfp

Mutation

F36M in FKBP

Available Since

May 19, 2008

Availability

Academic Institutions and Nonprofits only
CMVTO-KRAS-G12D

Plasmid

#179656

Purpose

KRAS active mutant (G12D mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12D

Expression

Mammalian

Mutation

G12D

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSIR-GFP

Plasmid

#51134

Purpose

Self-inactivating retrovirus vector containing the GFP gene

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-FKBP-nTEVP

Plasmid

#179675

Purpose

ER-associated FKBP split nTEVP (Rapalog inducible)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

FKBP-nTEVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLZL2386

Plasmid

#48682

Purpose

pET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)

Depositor

Suzanne Sandmeyer

Article

Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

Insert

PFV Integrase

Tags

His

Expression

Bacterial

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12V

Plasmid

#179654

Purpose

KRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12V

Expression

Mammalian

Mutation

G12V

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-mCherry-Kir2.1-HA-tevs-RXR

Plasmid

#179704

Purpose

TEVP-inducible mCherry-conjugated Kir2.1 RELEASE

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12C

Plasmid

#179655

Purpose

KRAS active mutant (G12C mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12C

Expression

Mammalian

Mutation

G12C

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSIR-hCD2

Plasmid

#51143

Purpose

Self-inactivating retrovirus vector containing the human CD2 gene

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pPTK005-3a-αMF

Plasmid

#84964

Purpose

Type 3a, α-mating factor - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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CMVTO-Gbp6-TM-Che

Plasmid

#180022

Purpose

Membrane-bound anti-GFP nanobody conjugated to mCherry

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Gpb6 nanobody

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-nTVMVp-AP4

Plasmid

#179669

Purpose

ER-associated uncaged-nTVMVP with AP4 leucine zipper

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

nTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-nTVMVp-hcvs-AP4

Plasmid

#179673

Purpose

HCVP-repressible ER-associated uncaged-nTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

nTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-hcvs-SpyCatcher

Plasmid

#179677

Purpose

HCVP-cleavable ER-associated SpyCatcher

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

HCVP SpyCatcher

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-Kir2.1-HA-tevs-RXR-V2

Plasmid

#179705

Purpose

TEVP-inducible GFP-conjugated Kir2.1 RELEASE

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-mCherry-Kir2.1-HA-tvmvs-RXR-V2

Plasmid

#179707

Purpose

TVMVP-inducible mCherry-conjugated Kir2.1 RELEASE

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-RARE-luciferase

Plasmid

#13458

Depositor

T. Michael Underhill

Article

Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

Insert

Retinoic Acid Receptor Response Element

Use

Luciferase

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pHCMV_Bless

Plasmid

#207255

Purpose

Mammalian expression of Baboon Endogenous Retrovirus env protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BaboonEndogenousRetrovirus_Rless_env

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pECFP18aaEYFP

Plasmid

#109330

Purpose

ECFP-EYFP FRET positive control

Depositor

Gabriele Kaminski Schierle

Article

Kaminski et al Methods Mol Biol. 2014;1076:445-54. doi:

Insert

ECFP-EYFP FRET positive control

Expression

Mammalian

Mutation

ECFP linked to EYFP with 18 aa linker GLRSRAQASNS…

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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CMVTO-nTVMVP-AP4-tevs-P3-tevs-cTVMVPmut

Plasmid

#179660

Purpose

Cytosolic TEVP-inducible caged-nTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged-nTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-nTVMVPmut-tevs-AP4-tevs-P3-cTVMVP

Plasmid

#179661

Purpose

Cytosolic TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged-cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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