1,878 results

1. pJW1806

   Plasmid

   #154319

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   linker::3xMyc for NT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJW1805

   Plasmid

   #154318

   Purpose

   CT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   linker::3xMyc for CT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJW1787

   Plasmid

   #154316

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJW1348

   Plasmid

   #154311

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker for NT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJW1822

   Plasmid

   #154322

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::GFP
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJW1594

   Plasmid

   #121061

   Purpose

   TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   TagRFP-T^SEC^BioTag::AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

   Expression

   Worm

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSDH51

   Plasmid

   #139045

   Purpose

   MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   halotag (no STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSM04

   Plasmid

   #139046

   Purpose

   MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   mkate2 (no STOP codon)
   

   Expression

   Worm

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSM10

   Plasmid

   #139034

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   mScarlet (no STOP codon)
   

   Expression

   Worm

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSM05

    Plasmid

    #139050

    Purpose

    MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    mkate2 (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSDH66

    Plasmid

    #139053

    Purpose

    MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    unc-54 3'UTR (unc-54 Nematode)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH2933

    Plasmid

    #132363

    Purpose

    Prgef-1 INS-3::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-3::GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    INS-3 (ins-3 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prgef-1

    Available Since

    Dec. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pEG584

    Plasmid

    #40191

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (RNAi resistant) (mex-5 Nematode)
    

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    Exon 2 recoded to be RNAi-resistant

    Promoter

    4.4 kb mex-5 promoter fragment

    Available Since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pAP639-2F

    Plasmid

    #99486

    Purpose

    3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag
    

    Expression

    Bacterial

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AP1297‐1

    Plasmid

    #87247

    Purpose

    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    

    Tags

    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMLS269

    Plasmid

    #73727

    Purpose

    SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)
    

    Use

    CRISPR

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOX-SK2

    Plasmid

    #16115

    Depositor

    Larry Salkoff

    Article

    Wei et al Neuropharmacology. 1996 . 35(7):805-29.

    Insert

    Ca2+-activated K+ channel proteins (kcnl-2 Nematode)
    

    Use

    Xenopus oocyte expression

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pGC267

    Plasmid

    #19618

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R1-R2)-iA-FLP::let-858(3’)-(Cbr)unc-119(+)
    

    Use

    Flp/frt

    Expression

    Worm

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBSc + Shal

    Plasmid

    #16142

    Depositor

    Larry Salkoff

    Article

    Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

    Insert

    Voltage-gated K+ channels (Y73B6BL.19 Nematode)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pISlin41m(s) (lin-41 microRNA mut reporter)

    Plasmid

    #11907

    Depositor

    David Bartel

    Article

    Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

    Insert

    lin-41 miRNA target sequence mut (lin-41 Nematode)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    point mutations in miRNA complementary site

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCFJ1259

    Plasmid

    #44479

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, peel-1, [4-3] Gateway
    

    Use

    Gateway destination vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJJR50

    Plasmid

    #75026

    Purpose

    U6 promoter driven flipped + extended sgRNA expression vector

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    guide RNA, flipped and extended version
    

    Use

    CRISPR

    Promoter

    R07E5.16 (U6)

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOX-twk43

    Plasmid

    #16128

    Depositor

    Larry Salkoff

    Article

    Salkoff et al Neuroscience. 2001 . 103(4):853-9.

    Insert

    Tandem pore domain K+ channel (F32H5.2, twk-43 Nematode)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pCM5.60

    Plasmid

    #19374

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    fog-1 3'utr in pDONRP2RP3
    

    Expression

    Worm

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCFJ1200

    Plasmid

    #44485

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, PuroR, peel-1, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJH2256

    Plasmid

    #132365

    Purpose

    Prgef-1 INS-6::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-6::GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    INS-6 (ins-6 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prgef-1

    Available Since

    Jan. 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. 6xHis-TEV-GEI-17(133-509)

    Plasmid

    #87056

    Purpose

    Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
    

    Tags

    6xHis-TEV and GAGGGS linker

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 6xHis-TEV-SMO-1(GG)

    Plasmid

    #87054

    Purpose

    Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    smo-1 (smo-1 Nematode)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    The last aa was removed to render processed SMO-1…

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBT3102

    Plasmid

    #122548

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a plasma membrane localized gene (aqp-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_aqp-1::CemOrange2 (aqp-1 Nematode, Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. U371-GFP

    Plasmid

    #24476

    Depositor

    Ron Vale

    Article

    Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

    Insert

    Unc104 (unc-104 Nematode)
    

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Contains only the kinesin's motor head fused…

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only