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Showing: 1451 - 1500 of 32226 results

  3. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Expression
    Mammalian
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

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  6. Td-CGBE

    Plasmid
    #196599
    Purpose
    Mammalian expression of Td-CGBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L); S. pyogenes Cas9 (D10A)
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196599-plasmid-map-sequence-id-386792

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  8. PA-Dre

    Plasmid
    #169706
    Purpose
    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells
    Depositor
    Dali Li
    Article
    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11
    Insert
    Dre246-nMag-T2A-pMag-Dre247
    Use
    AAV
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CAG
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    169706-plasmid-map-sequence-id-333284

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  9. CALID

    Plasmid
    #169708
    Purpose
    Cre-activated light-inducible Dre recombinase
    Depositor
    Dali Li
    Article
    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11
    Insert
    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CAG
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    169708-plasmid-map-sequence-id-333329

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  10. gNS2B47NS3 S135A

    Plasmid
    #155317
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155317-plasmid-map-sequence-id-311234

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  15. eNS2B47NS3 S135A

    Plasmid
    #155318
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155318-plasmid-map-sequence-id-311249

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  16. pM-ABE

    Plasmid
    #196292
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding ABE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196292-plasmid-map-sequence-id-390025

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  17. pS-sg:GFP

    Plasmid
    #196296
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196296-plasmid-map-sequence-id-389821

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  18. pM-Cas9

    Plasmid
    #196325
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196325-plasmid-map-sequence-id-390015

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  20. pTK Cas9-2A-Citrine

    Plasmid
    #92393
    Purpose
    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    Cas9 2A Citrine
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    thymidine kinase promoter
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92393-plasmid-map-sequence-id-190866

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  21. pM-Cas12a

    Plasmid
    #196291
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    Use
    CRISPR
    Tags
    Flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196291-plasmid-map-sequence-id-389824

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  23. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

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  26. Td-CBEmax

    Plasmid
    #196600
    Purpose
    Mammalian expression of Td-CBEmax
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196600-plasmid-map-sequence-id-386793

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  27. eNS2B47NS3 L30SF31S

    Plasmid
    #155319
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    L30 to S, F31 to S
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155319-plasmid-map-sequence-id-311251

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  31. gNS2B47NS3 L30SF31S

    Plasmid
    #155316
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    L30 to S, F31 to S
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155316-plasmid-map-sequence-id-311228

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  44. peSCBE3-NG

    Plasmid
    #218163
    Purpose
    This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218163-plasmid-map-sequence-id-433173

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  45. pSCBE3-NG

    Plasmid
    #218159
    Purpose
    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / L1111…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218159-plasmid-map-sequence-id-433199

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  50. pSCBE3-HF1

    Plasmid
    #218156
    Purpose
    This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218156-plasmid-map-sequence-id-434901

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Showing: 1451 - 1500 of 32226 results