We narrowed to 44,068 results for: cha

1. pcDNA6-rbN1 S77A

   Plasmid

   #32945

   Depositor

   Edward Weinman

   Article

   Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

   Insert

   SLc9a3r1 (SLC9A3R1 rabbit)
   

   Use

   Tags

   T7 tag, his, and partial FLAG

   Expression

   Mammalian

   Mutation

   Change Serine77 to Alanine

   Promoter

   CMV

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA6-rbN1 S77D

   Plasmid

   #32947

   Depositor

   Edward Weinman

   Article

   Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

   Insert

   slc9a3r1 (SLC9A3R1 rabbit)
   

   Use

   Tags

   T7 tag, his, and partial FLAG

   Expression

   Mammalian

   Mutation

   Serine 77 mutated to Aspartic Acid

   Promoter

   CMV

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPomyces-2BD

   Plasmid

   #234428

   Purpose

   Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified Cas9 with polyaspartate linking
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Promoter

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCRISPomyces-2BD

   Plasmid

   #234429

   Purpose

   Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified dCas9(D10A, H840A) with polyaspartate linking
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Promoter

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_rtTA-P2A-BFP_HygroR

   Plasmid

   #186738

   Purpose

   rtTA-P2A-BFP expression construct cloned into lentiviral backbone

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   rtTA
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR-v2-FE-PuroR

   Plasmid

   #186746

   Purpose

   Modified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpin

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pROS13_IDH2 #7

   Plasmid

   #166102

   Purpose

   This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH2 sgRNA #7 (IDH2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pROS13_IDH1 #4

   Plasmid

   #166101

   Purpose

   This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH1 sgRNA #4 (IDH1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiTet-P2A-mCherry BlastR

   Plasmid

   #186743

   Purpose

   Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   P2A-mcherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiTet-OTX2-P2A-mCherry BlastR

    Plasmid

    #186741

    Purpose

    Dox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    OTX2 (OTX2 Human)
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGIPZ-PD-L1-EGFP

    Plasmid

    #120933

    Purpose

    Express PD-L1-EGFP fusion protein in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

    Insert

    PD-L1-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO-Tet-On-shRab27a

    Plasmid

    #120930

    Purpose

    conditionally knockdown Rab27a in mouse cell lines

    Depositor

    Mien-Chie Hung

    Article

    Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

    Insert

    shRab27a (Rab27a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEYFP-C1-hSERT

    Plasmid

    #70103

    Purpose

    mammalian expression of hSERT with N-terminal YFP fusion

    Depositor

    Harald Sitte

    Article

    Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

    Insert

    SERT (SLC6A4 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pECFP-C1-hSERT

    Plasmid

    #70104

    Purpose

    mammalian expression of hSERT with N-terminal CFP fusion

    Depositor

    Harald Sitte

    Article

    Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

    Insert

    SERT (SLC6A4 Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

    Plasmid

    #186745

    Purpose

    dCas9-10XGcn4-P2A-mCherry expression construct

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    Cas9-10XGcn4-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiTet-LDLR-mCherry BlastR

    Plasmid

    #186739

    Purpose

    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    LDLR (LDLR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGIPz-mPD-L1

    Plasmid

    #121488

    Purpose

    Expression of mouse PD-L1 WT

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-flag-hKDM4A-polyA

    Plasmid

    #101051

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-flag-hKDM4A(H188A)-polyA

    Plasmid

    #101052

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG416GALL-ccdB-6stop

    Plasmid

    #203511

    Purpose

    Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge