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pIRES2-eGFP:HA-mTREK-1(tandem S131C-wt)

Plasmid

#224777

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S131C

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(S131C/A286F)

Plasmid

#224775

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S131C / A286F

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)

Plasmid

#224778

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 wt-S131C) (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S562C

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)

Plasmid

#224776

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-S131C)

Plasmid

#224760

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C / S562C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131A/C159A/C219A)

Plasmid

#224753

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131A/C159A/C219A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131C/C159A/C219A)

Plasmid

#224752

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C/C159A/C219A

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)

Plasmid

#224754

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

C159A/C219A/C365A/C399A

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC1_oROS-HT_LF(C199S)

Plasmid

#216412

Purpose

Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT_LF(C199S)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-lifeact-oROS-HT

Plasmid

#216420

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

lifeact-oROS-HT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT_WPRE

Plasmid

#216414

Purpose

Encodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

Plasmid

#178857

Purpose

Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

Plasmid

#178858

Purpose

Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR207-MUM2sp-Citrine-ADPG2

Plasmid

#170725

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)

Use

Gateway donor vector / entry clone

Tags

Citrine tag (714bp)

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR207-MUM2sp-ADPG2

Plasmid

#170723

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)

Use

Gateway donor vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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XLone-Puro Cas13d-eGFP U6 RUNX1 g2

Plasmid

#155186

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA2

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

Cas13d RUNX1 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 SOX17 g1

Plasmid

#155187

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA1

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

Cas13d SOX17 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2(delta)TK-VSV

Plasmid

#139353

Purpose

Expression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)

Depositor

Sandra Pellegrini

Article

Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

Insert

TYK2 cDNA expressing aa 1-895 (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F-deletion of aa 896-1187

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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AF10_OM-LZ

Plasmid

#111753

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

MLLT10 (MLLT10 Human)

Use

Tags

6x His - TEV

Expression

Bacterial

Mutation

Contains the OM-LZ region of AF10 (residues 720-7…

Promoter

T7

Available since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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LP697

Plasmid

#90178

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1650-1826

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1650-1826

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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