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pAAV-hSyn-mem-seTurboID

Plasmid

#237888

Purpose

AAV vector encoding membrane-tethered seTurboID under the control of human synapsin promoter

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

mem-seTurboID

Use

AAV

Tags

GAP43 palmitoylation signal and V5 tag

Expression

Mammalian

Promoter

human Synapsin 1 (hSyn)

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-seTurboID-IRES-tTA

Plasmid

#240206

Purpose

Amplifier AAV vector of Dual-AAV sparse labeling system encoding seTurboID

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

seTurboID

Use

AAV

Tags

FLAG tag

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGTPA-lb

Plasmid

#171528

Purpose

Plasmid contains the Cas12k gRNA for mutant library construction

Depositor

Quanjiang Ji

Article

Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

Insert

Cas12k gRNA

Use

CRISPR

Promoter

Constitutive promoter J23119

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGTPA

Plasmid

#171525

Purpose

Plasmid contains the Cas12k gRNA and transposon donor cassette

Depositor

Quanjiang Ji

Article

Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

Insert

Cas12k gRNA, transposon donor cassette

Use

CRISPR

Promoter

Constitutive promoter J23119

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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2BT-His-TEV-cs-LC3b

Plasmid

#197460

Purpose

Bacterial Expression of human LC3b

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

LC3b (MAP1LC3B Human)

Tags

His

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-IG-ATG3 (H262A)

Plasmid

#212027

Purpose

Expresses untagged ATG3 (H262A) in mammalian cells

Depositor

Michael Lazarou

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro

Plasmid

#210855

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

V44D/V314D

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-V314D-mCherry-Hygro

Plasmid

#210854

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

V314D

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-V44D-mCherry-Hygro

Plasmid

#210853

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

V44D

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG101

Plasmid

#210858

Purpose

Mammalian expression of ATG101

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG101 (ATG101 Human)

Expression

Mammalian

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

Plasmid

#210842

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Expression

Mammalian

Mutation

L324D/R543D/R548D

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1B (137-423)

Plasmid

#198172

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1B (137-423)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP

Plasmid

#171838

Purpose

Mammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

H85E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP

Plasmid

#171839

Purpose

Mammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

K88E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dI92E-cs-TEV-STREP

Plasmid

#171840

Purpose

Mammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

I92E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dC93E-cs-TEV-STREP

Plasmid

#171841

Purpose

Mammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

C93E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP

Plasmid

#171843

Purpose

Mammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

R125E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

Plasmid

#171844

Purpose

Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

K128E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D977A

Plasmid

#129674

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

D977A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ290-E848A

Plasmid

#129675

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Tags

E848A; AacCas12b is rice codon optimized

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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