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Plasmid#97328PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T2
UseTags6xHis - TEVExpressionBacterialMutationAddition of two T residues after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7CNW)RS
Plasmid#222874PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 HCoV-229E N_stop
Plasmid#168768PurposeGateway-compatible Entry vectorDepositorInsertN (N )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP6_stop
Plasmid#168753PurposeGateway-compatible Entry vectorDepositorInsertNSP6 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP7_stop
Plasmid#168754PurposeGateway-compatible Entry vectorDepositorInsertNSP7 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP8_stop
Plasmid#168755PurposeGateway-compatible Entry vectorDepositorInsertNSP8 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP10_stop
Plasmid#168757PurposeGateway-compatible Entry vectorDepositorInsertNSP10 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP12_stop
Plasmid#168758PurposeGateway-compatible Entry vectorDepositorInsertNSP12 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP13_stop
Plasmid#168759PurposeGateway-compatible Entry vectorDepositorInsertNSP13 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP14_stop
Plasmid#168760PurposeGateway-compatible Entry vectorDepositorInsertNSP14 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP15_stop
Plasmid#168761PurposeGateway-compatible Entry vectorDepositorInsertNSP15 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E S_stop
Plasmid#168763PurposeGateway-compatible Entry vectorDepositorInsertS (S )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E ORF4a_stop
Plasmid#168764PurposeGateway-compatible Entry vectorDepositorInsertORF4a (4a )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E ORF4b_stop
Plasmid#168765PurposeGateway-compatible Entry vectorDepositorInsertORF4b (4b )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E E_stop
Plasmid#168766PurposeGateway-compatible Entry vectorDepositorInsertE (E )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E M_stop
Plasmid#168767PurposeGateway-compatible Entry vectorDepositorInsertM (M )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP9_nostop
Plasmid#168736PurposeGateway-compatible Entry vectorDepositorInsertNSP9 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP10_nostop
Plasmid#168737PurposeGateway-compatible Entry vectorDepositorInsertNSP10 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 HCoV-229E NSP12_nostop
Plasmid#168738PurposeGateway-compatible Entry vectorDepositorInsertNSP12 (HCoV229Egp1 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits