178,748 results
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Plasmid#169247PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianPromoterhSynAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
spNW50
Plasmid#173486PurposeExpresses spNW50 in Bl21DepositorInsertspNW50
TagsHistagExpressionBacterialAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)
Plasmid#163056Purposemito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)DepositorInsertmito-roGFP2-Grx1
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE
Plasmid#162377PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas serviceAAV1, AAV9, and AAV RetrogradeInsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-SpCas9-P2A-EGFP (RTW3027)
Plasmid#139987PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT076
Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
Plasmid#134867PurposeExpresses EGFP-tagged nuclear envelope markerDepositorAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0079 RESCUE-S
Plasmid#130662PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16SDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdRanCas13b-ADAR2dd
ExpressionMammalianAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mito-mEosEM
Plasmid#132706Purposeapplication in Correlative light-electron microscopyDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmitochondria targeting sequence
TagsmEosEMExpressionMammalianPromoterCMVAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSH-EFIRES-P-AtAFB2
Plasmid#129715PurposeAAVS1 safe harbor targeting vector expressing AtAFB2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsnoExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TEVSH
Plasmid#125194PurposeHis-tagged TEV protease with solubility increasing mutationsDepositorInsertTEV_SH
TagsV5 His-tagExpressionBacterialPromoterT7lacAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry- SEC61B
Plasmid#121160PurposeMammalian expression of mCherry tagged SEC61B, used as a general ER markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEC61B (SEC61B )
ExpressionMammalianAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR OVA/p2a/mCherry-CaaX
Plasmid#113030Purposefor lentivirus production of ovalbumin and mcherry-caaxDepositorInsertOVA/p2a/mCherry-CaaX
UseLentiviralTagsmCherryExpressionMammalianAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_DA1h
Plasmid#113050Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
UseAAVPromoterhSynAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKC-4.06
Plasmid#112084PurposeNMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3XFLAG firefly luciferase-beta-globin 39PTC (HBB Mouse, Photinus pyralis, Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Artichoke
Plasmid#73320PurposeArtichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CWB-yellow pre-eGRASP(p32)
Plasmid#111580PurposeAn AAV vector that expresses yellow pre-eGRASP(p32) under the CaMKIIa promoter.DepositorInsertyellow pre-eGRASP(p32)
UseAAVPromoterCaMKIIaAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ELONGIN BC (XX01TCEB1A-c001)
Plasmid#110274PurposeElongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin CDepositorExpressionBacterialMutationElongin B is full length. Elongin C contains a.a…PromoterT7Available sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCherry.90 alpha
Plasmid#108222PurposeMammalian expression vector for mCherry fusion to human Hsp90 alphaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-Hsp90a (HSP90AA1 Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPBbsr2-4031NES
Plasmid#105241PurposeA Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK-EV
ExpressionMammalianPromoterCAGGSAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by