We narrowed to 18,228 results for: gateway

1. pDL1-eGFP

   Plasmid

   #242945

   Purpose

   Encodes a fragment of eGFP for generating dsRNA for use as a control in RNAi experiments

   Depositor

   Erin Davies

   Article

   Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

   Insert

   eGFP
   

   Use

   RNAi

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCM167-VenusFlag-hDHX58

   Plasmid

   #241879

   Purpose

   Expresses VenusFlag-hDHX58 in mammalian cells

   Depositor

   Haian Fu

   Insert

   DHX58 (DHX58 Human)
   

   Tags

   Venus-Flag

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG426-GAL-EWSR1

   Plasmid

   #181725

   Purpose

   Galactose inducible expression of EWSR1

   Depositor

   Alejandro Chavez

   Insert

   EWSR1 (EWSR1 Human)
   

   Expression

   Yeast

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPI-Glis3M412

   Plasmid

   #216854

   Purpose

   This plasmid is for the production of lentiviral vectors expressing an active form of Glis3 together with GFP.

   Depositor

   Patrick Salmon

   Insert

   Glis3 (Glis3 Mouse)
   

   Use

   Lentiviral

   Mutation

   N-terminal deletion removing repressor domains

   Promoter

   EF1 alpha

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. N-SF-TAP-DEST-TLK2-D592V

   Plasmid

   #218835

   Purpose

   Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).

   Depositor

   Travis Stracker

   Insert

   TLK2 Q592V (kinase dead) (TLK2 Human)
   

   Tags

   FLAG-STREP

   Expression

   Mammalian

   Mutation

   Q592V

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. nTALECD for_TUB4S351F_1397NC

   Plasmid

   #193382

   Purpose

   nTALECD (1397NC) expression vector

   Depositor

   Shin-ichi Arimura

   Article

   Hosoda et al bioRxiv 2023.02.27.530312

   Insert

   For specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtTUB4.
   

   Expression

   Plant

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EV-D68 2A protease; no tag, low expression levels

   Plasmid

   #204805

   Purpose

   Bacterial expresison of Enterovirus D68 2A protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   D68 2A protease
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDEST27-GST-hSFRP1(aa181-314)

   Plasmid

   #189548

   Purpose

   Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.

   Depositor

   Haian Fu

   Insert

   truncated secreted frizzled related protein 1 (SFRP1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   contains aa181-314

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST27-GST-hSFRP1(aa53-169)

   Plasmid

   #189545

   Purpose

   Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.

   Depositor

   Haian Fu

   Insert

   truncated secreted frizzled related protein 1 (SFRP1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   contains aa53-169

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWT35S-Citrine

    Plasmid

    #182581

    Purpose

    Transient expression of Citrine in plant cell (Cytosol)

    Depositor

    Yutaka Kodama

    Article

    Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

    Insert

    Citrine
    

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCell-Free_KA1_SARS-COV-2_S-frag3

    Plasmid

    #169403

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    N-term frag. S protein after the furin cleavag (N SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCellFree_KA1_SARS-COV-2_S-frag5

    Plasmid

    #169404

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    C-term frag. S protein after the furin cleavag (S SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GGDest1-Amp

    Plasmid

    #157649

    Purpose

    Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

    Depositor

    Karmella Haynes

    Article

    Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Promoter

    none

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMYA5tnC/pDEST26-N-FLAG

    Plasmid

    #98130

    Purpose

    Express CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vector

    Depositor

    Rita Shiang

    Insert

    CMYA5 (CMYA5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGC244

    Plasmid

    #19672

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Use

    Flp/frt

    Available Since

    Feb. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX-EF1a_GFP_CdTag-HA

    Plasmid

    #221492

    Purpose

    GFP lentiviral overexpression of C-terminal dTag fusion

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG416Gal-GFP

    Plasmid

    #38309

    Depositor

    Susan Lindquist

    Article

    McLellan et al ACS Chem Biol. 2012 Jun 22.

    Insert

    EGFP (eGFP )
    

    Expression

    Yeast

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pLEX307-APN-puro

    Plasmid

    #158454

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-USP1

    Plasmid

    #22596

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP1 (USP1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX307-APN-blast

    Plasmid

    #158455

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFRT-TODestFLAGHA_HuC

    Plasmid

    #65756

    Purpose

    expresses FLAGHA-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFRT-TODestFLAGHA_HuD

    Plasmid

    #65757

    Purpose

    expresses FLAGHA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFRT-TODestFLAGHA_HuB

    Plasmid

    #65755

    Purpose

    expresses FLAGHA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pKOR1

    Plasmid

    #133446

    Purpose

    Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

    Depositor

    Olaf Schneewind, Taeok Bae

    Article

    Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

    Type

    Empty backbone

    Use

    E.coli/s.aureus shuttle vector

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. dg263_pENTR_ChrimsonCel

    Plasmid

    #66101

    Purpose

    Entry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chrimson
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. dg265_pENTR_CoChRCel

    Plasmid

    #66103

    Purpose

    Entry clone containing CoChR coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    CoChR
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. dg264_pENTR_ChronosCel

    Plasmid

    #66102

    Purpose

    Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chronos
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDEST17-UbE2E2

    Plasmid

    #15788

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2E2 (UBE2E2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

29. Flag-HA-USP30

    Plasmid

    #22578

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP30 (USP30 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLARRY-EGFP

    Plasmid

    #140025

    Purpose

    Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

    Depositor

    Fernando Camargo

    Article

    Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    EF1alpha

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge