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We narrowed to 18,759 results for: nls

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Showing: 11961 - 12000 of 18759 results

  1. AAVS1 LAMP1-mNeonGreen HRD

    Plasmid
    #207549
    Purpose
    Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells
    Depositor
    Jens Schmidt
    Publication
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET inducible LAMP1-mNeonGreen
    Tags
    mNeonGreen
    Expression
    Mammalian
    Promoter
    Endogenous
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207549-plasmid-map-sequence-id-430997

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  2. AAVS1 EGFP-LC3B HRD

    Plasmid
    #207551
    Purpose
    Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells
    Depositor
    Jens Schmidt
    Publication
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET inducible EGFP-LC3B
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    Endogenous
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207551-plasmid-map-sequence-id-430996

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  3. AAVS1 EGFP-GABARAPL1 HRD

    Plasmid
    #207552
    Purpose
    Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells
    Depositor
    Jens Schmidt
    Publication
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET inducible EGFP-GABARAPL1
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    Endogenous
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207552-plasmid-map-sequence-id-430995

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  4. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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  5. MDC1 KO sgRNA

    Plasmid
    #207103
    Purpose
    pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGTGTAACGTGGAGCCAGTA
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207103-plasmid-map-sequence-id-430975

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  6. 3xFLAG-dCas9/pMSCVneo

    Plasmid
    #134982
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134982-plasmid-map-sequence-id-430627

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  7. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  10. 2xHP1bCD-IFP2.0C

    Plasmid
    #215750
    Purpose
    Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors
    Depositor
    Albert Jeltsch
    Publication
    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xHP1bCD-IFP2.0C
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    215750-plasmid-map-sequence-id-429725

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  11. AID-C12*-BE4max

    Plasmid
    #216729
    Purpose
    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.
    Depositor
    Rahul Kohli
    Publication
    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-C12*-BE4max (AICDA Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    216729-plasmid-map-sequence-id-429719

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  12. CCND2 targeting gRNA

    Plasmid
    #215318
    Purpose
    Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.
    Depositor
    Michele Pagano
    Publication
    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215318-plasmid-map-sequence-id-429221

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  22. GBH-2aG4-gcryBFP.3

    Plasmid
    #188015
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188015-plasmid-map-sequence-id-380747

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  23. GBH-R-gcryBFP.1

    Plasmid
    #187995
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    187995-plasmid-map-sequence-id-380758

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  24. GBH-R-gcryGFP.3

    Plasmid
    #188000
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188000-plasmid-map-sequence-id-381121

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  26. pCMV-RVR-A3A-Y130F

    Plasmid
    #193658
    Purpose
    Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RVR-dLbCas12a-hA3A-Y130F, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    193658-plasmid-map-sequence-id-426532

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  35. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid
    #214125
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-TET
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214125-plasmid-map-sequence-id-424237

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  39. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid
    #191388
    Purpose
    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio
    Depositor
    Yannick Doyon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hGEM(1-110)_hSLBP(56_108)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191388-plasmid-map-sequence-id-378110

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  40. pCMV-RVR-A3A-N57G

    Plasmid
    #193657
    Purpose
    Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RVR-dLbCas12a-hA3A-N57G, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193657-plasmid-map-sequence-id-422142

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Showing: 11961 - 12000 of 18759 results