We narrowed to 32,142 results for: eng

1. pCAGIG-FLAG-SHTN1LRRR>GGG

   Plasmid

   #209570

   Purpose

   Mammalian expression of Shtn1 long isoform with RG mutation

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)
   

   Expression

   Mammalian

   Mutation

   RRR to GGG

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Coexpressed VLP NES

   Plasmid

   #182433

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Coexpressed VLP NES (M)

   Plasmid

   #182435

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(M)
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Coexpressed VLP NES (G)

   Plasmid

   #182477

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(G)
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Donor_C_FH_VIR

   Plasmid

   #186660

   Purpose

   Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Donor_N_FH_Shu

   Plasmid

   #186655

   Purpose

   Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Donor_C_FH_Nbr

   Plasmid

   #186662

   Purpose

   Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDonor_N_FH_GLE

   Plasmid

   #186657

   Purpose

   Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV V5-Chpt1

   Plasmid

   #175155

   Purpose

   Lentiviral expression of V5-tagged mouse Chpt1

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Chpt1 (Chpt1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV Dnajc16-V5

    Plasmid

    #175156

    Purpose

    Lentiviral expression of mouse Dnajc16-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Dnajc16 (Dnajc16 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV Cdipt-V5

    Plasmid

    #175144

    Purpose

    Lentiviral expression of mouse Cdipt-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Cdipt (Cdipt Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3xFLAG-CMV-Drosha(W1258)

    Plasmid

    #171535

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Expression

    Mammalian

    Mutation

    a Drosha stopping at W1258

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV Myc-emerin

    Plasmid

    #175101

    Purpose

    Lentiviral expression of Myc-tagged mouse Emd

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Emd (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3xFLAG-CMV-Drosha (P426)

    Plasmid

    #171537

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Expression

    Mammalian

    Mutation

    a Drosha mutant starting from P426

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3xFLAG-CMV-Drosha (E419)

    Plasmid

    #171534

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Expression

    Mammalian

    Mutation

    a Drosha mutant stopping at E419

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCK-Drosha-FLAG(delta6)

    Plasmid

    #171532

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Expression

    Mammalian

    Mutation

    small deletions of Drosha_1271A to 1276G_

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-1-Dicer-dsRBD (+6)

    Plasmid

    #171544

    Purpose

    Encodes a Dicer fragment extending from from R1841 to the C-terminus

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    DICER1 (DICER1 Human)
    

    Expression

    Bacterial

    Mutation

    a six amino acid_TRLTEG_insertion in the L1 region

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-Tac-STC

    Plasmid

    #162490

    Purpose

    Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its stem region, TM…

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. BE3-YE1

    Plasmid

    #132943

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    BE3-(W90Y-R126E)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    BE3-(W90Y-R126E)

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AdMKP-3 S159AS197A

    Plasmid

    #131523

    Purpose

    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    changed Serine 159 to Alanine and Serine 197 to A…

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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