We narrowed to 7,293 results for: tet

1. pAXEF-Rap1-WT

   Plasmid

   #32697

   Depositor

   Vicki Boussiotis

   Article

   Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

   Insert

   RAP1 (RAP1A Human)
   

   Expression

   Mammalian

   Promoter

   EF promoter

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-TRE-DIO-ChR2-TdTomato

   Plasmid

   #166600

   Purpose

   Encodes Cre-dependent ChR2-TdTomato under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   ChR2-TdTomato
   

   Use

   AAV

   Mutation

   H134R

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK02

   Plasmid

   #105133

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAR357_SFP_pCDF-1b

   Plasmid

   #122851

   Purpose

   Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   Sfp
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. qi5

   Plasmid

   #24501

   Depositor

   Bruce Conklin

   Article

   Conklin et al Nature. 1993 May 20. 363(6426):274-6.

   Insert

   Gq alpha (Gnaq Mouse)
   

   Tags

   HA (see comments)

   Expression

   Mammalian

   Mutation

   Gq alpha with the C-terminal amino acids changed …

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-TRE-DIO-Cas9

   Plasmid

   #166601

   Purpose

   Encodes Cre-dependent Cas9 under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   Cas9
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP.R270

   Plasmid

   #138219

   Purpose

   Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP.R269

    Plasmid

    #138181

    Purpose

    Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFP.R268

    Plasmid

    #138180

    Purpose

    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDN-Sth1Cas9

    Plasmid

    #221386

    Purpose

    Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

    Depositor

    Deborah Hung

    Article

    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

    Insert

    Sth1 Cas9
    

    Use

    CRISPR

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. RNA-Protein Landing Pad

    Plasmid

    #226162

    Purpose

    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ131-tRNA2.0

    Plasmid

    #158393

    Purpose

    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Without promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. fos-tTA

    Plasmid

    #34856

    Depositor

    Mark Mayford

    Article

    Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

    Insert

    Fos promoter/first intron -764/+918 (Fos Mouse)
    

    Use

    Mouse Targeting

    Tags

    Fos 3' UTR (~1500bp) and tTA (tetracycline-c…

    Expression

    Mammalian

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. GPR_control_lenti

    Plasmid

    #216223

    Purpose

    GFP P2A RFP control reporter in a lenti backbone

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_stop
    

    Expression

    Mammalian

    Available Since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TRE-DIO-TVA-oG-mCherry

    Plasmid

    #166602

    Purpose

    Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    TVA-oG-mCherry
    

    Use

    AAV

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MXS_CMV::rtTA3-bGHpA

    Plasmid

    #62445

    Purpose

    MXS_chaining vector with CMV::rtTA3-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the reverse tetracycline transactivator with CMV promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBO.ACT001

    Plasmid

    #182711

    Purpose

    aTc inducible dCasRx

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    2x-FLAG

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLD3 translation factors

    Plasmid

    #117762

    Purpose

    BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 22-30
    

    Use

    Escherichia coli

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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