We narrowed to 19,565 results for: Cre

1. pNH090

   Plasmid

   #42166

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   mTFP1::[(G4S)3]::C-TAP Tag
   

   Available Since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. KHBD00420

   Plasmid

   #34527

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG11301
   

   Use

   Gateway donor vector

   Available Since

   Jan. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pENTR APLP1 shRNA 3

   Plasmid

   #30158

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

   Insert

   APLP1 shRNA 3
   

   Expression

   Mammalian

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

4. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiV2-EF1α-nCas9/RT

   Plasmid

   #210188

   Purpose

   Expression of nCas9/RT-P2A-Puro

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   nCas9/RT-P2A-Puro
   

   Use

   Lentiviral

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-LMNA-gRNA1

   Plasmid

   #122507

   Purpose

   expresses WT spCas9 and a chimeric gRNA targeting human LMNA

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   LMNA-gRNA1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-hEF1A-MG-U6-sgRNA

   Plasmid

   #175505

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hEF1a, U6

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CT3G-ERP2

   Plasmid

   #175497

   Purpose

   All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Str-KDEL_flGalT-SBP-mCherry

    Plasmid

    #65273

    Purpose

    synchronize trafficking of GalT from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    galactosidase
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. eGFP-TRF1 pWzl-Hygro

    Plasmid

    #19834

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

    Insert

    Telomere-Repeat binding Factor 1 (Terf1 Mouse)
    

    Use

    Retroviral

    Tags

    enhanced GFP

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pLC-ZsGreen-P2A-Puro

    Plasmid

    #124302

    Purpose

    Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    ZsGreen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Wnt8A-V5

    Plasmid

    #35935

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt8A (Wnt8a Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Silent Mutation A (600) --> G

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_174

    Plasmid

    #136476

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    EnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Promoter

    pCMV-EGFP

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNZ18-CAH

    Plasmid

    #203159

    Purpose

    Replicates in E. coli, S. cerevisiae, and A. laidlawii

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ML1Nwt_in_pEGFP-C1

    Plasmid

    #67797

    Purpose

    Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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