We narrowed to 18,937 results for: Tec

1. pAPcrRNA_Lmo

   Plasmid

   #126501

   Purpose

   L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC61

   Plasmid

   #118877

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)

   Plasmid

   #191162

   Purpose

   Expression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry output

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mTagBFP:s70587:mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC28

   Plasmid

   #118873

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEL033

   Plasmid

   #137902

   Purpose

   STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA345

   Plasmid

   #216031

   Purpose

   Fragmid fragment: (Cas protein) Cas13d

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas13d_v1.1 [Dj]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SNF-5 pXOOM

   Plasmid

   #48697

   Purpose

   Expressed SNF-5 in Xenopus oocytes or mammalian cells

   Depositor

   Dmitri Boudko

   Article

   Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

   Insert

   snf-5 (snf-5 Nematode)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Mutation

   K42E (when compared to NP_496735.1)

   Promoter

   T7

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_834

   Plasmid

   #216087

   Purpose

   Cas9 [Sp] knockout targeting CD59, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD59 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sg-mGreenLantern/EGFP

   Plasmid

   #179913

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-globin_del5UTR_WT-xrRNA-4H

    Plasmid

    #108366

    Purpose

    Expresses wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEL029

    Plasmid

    #137898

    Purpose

    STU2.0 SpyCas9 RZ system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

    Plasmid

    #169531

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. shNTC

    Plasmid

    #73549

    Purpose

    Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shNTC
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1(+)-sapphireAKAR-NLS

    Plasmid

    #118467

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR-NLS
    

    Tags

    Nuclear localization signal (NLS)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2SCb

    Plasmid

    #59385

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat2
    

    Use

    Template

    Mutation

    The 3’-end of the chloramphenicol resistance gene…

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57mini ZM249M P90A _env eGFP

    Plasmid

    #132939

    Purpose

    pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    HIV-1ZM249M provirus (P90A) with env deletion, GFP
    

    Use

    Lentiviral

    Mutation

    P90A

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB421

    Plasmid

    #74815

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. p168

    Plasmid

    #62618

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

    Plasmid

    #169534

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized yellow fluorescent protein Ypet
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. TAL2302

    Plasmid

    #36731

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA2-TALEN_Left (Brca2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only