We narrowed to 11,748 results for: pcdna

1. PME-1 S156A (inakt.mut) pcDNA-StrepIII

   Plasmid

   #119293

   Purpose

   Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active center

   Depositor

   Jukka Westermarck

   Insert

   PPME1 (PPME1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S156A

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYY166 pcDNA3.4-His-MBP-hAID

   Plasmid

   #231942

   Purpose

   Expression of His-MBP-AID fusion protein in mammalian cells

   Depositor

   Fei-Long Meng

   Article

   Wang et al Cell. 2023 May 11;186(10):2193-2207.e19.

   Insert

   AID (AICDA Human)
   

   Use

   Tags

   His-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT to BioID-nDIC2-3xFlag

   Plasmid

   #232418

   Purpose

   Expresses human dynein subunit DIC2 in mammalian cells.

   Depositor

   Samara Reck-Peterson

   Article

   Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

   Insert

   Dynein Intermediate Chain 2 (DYNC1I2 Human)
   

   Use

   Tags

   BioID and Flag-3x

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA FRT to BioID-cDIC1-3xFlag

   Plasmid

   #232417

   Purpose

   Expresses human dynein subunit DIC1 in mammalian cells.

   Depositor

   Samara Reck-Peterson

   Article

   Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

   Insert

   Dynein Intermediate Chain 1 (DYNC1I1 Human)
   

   Use

   Tags

   BioID and Flag-3x

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

   Plasmid

   #233064

   Purpose

   Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

   Plasmid

   #233063

   Purpose

   Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Threonine 923 to Glycine

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Cas9-P2A-dTomato-T20

   Plasmid

   #231912

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5 FRT to BioID-cDIC2-3xFlag

   Plasmid

   #232416

   Purpose

   Expresses human dynein subunit DIC2 in mammalian cells.

   Depositor

   Samara Reck-Peterson

   Article

   Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

   Insert

   Dynein Intermediate Chain 2 (DYNC1I2 Human)
   

   Use

   Tags

   BioID and Flag-3x

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 T21R-Nb139-T2A-mCherry

    Plasmid

    #225589

    Purpose

    Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-Nb139-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3puro-mGFP-FXR1a-CC1mut (N202P)

    Plasmid

    #225455

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a (N202P)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N202P

    Promoter

    CMV

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, R68A, R97A, K98A)

    Plasmid

    #231379

    Purpose

    Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

    Depositor

    David Bartel

    Article

    Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

    Insert

    Human Argonaute2 (AGO2 Synthetic, Human)
    

    Use

    Tags

    3xFLAG-SUMO_Eu1

    Expression

    Mammalian

    Mutation

    H56A, R68A, R97A, K98A

    Promoter

    CMV

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

    Plasmid

    #228532

    Purpose

    A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HA-GTSE1-S91, 262 ,724A

    Plasmid

    #224737

    Purpose

    For expression of HA-tagged GTSE1 protein with S91, 262 ,724A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 91, 262 ,724 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_eGFP-pScaC-MTS

    Plasmid

    #228827

    Purpose

    For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    MTS and eGFP

    Expression

    Mammalian

    Mutation

    aa 33-223

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3puro-EGFP-FXR1-C (379-621)

    Plasmid

    #225451

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a (FXR1 fragment (379-621))

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    C terminal fragment (379-621)

    Promoter

    CMV

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)

    Plasmid

    #227614

    Purpose

    Overexpression of SLC25A46 in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

    Plasmid

    #227619

    Purpose

    Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    deleted aa 2-83

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

    Plasmid

    #209411

    Purpose

    Expression of wild-type adult isoform of human SCN8A.

    Depositor

    Al George

    Article

    Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

    Insert

    SCN8A Variant 3, stabilized (SCN8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Modifed human HSPA5 intron 4 inserted after bp237…

    Promoter

    CMV

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3-FLAG-Regnase-3-HA_full-length

    Plasmid

    #222656

    Purpose

    Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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