We narrowed to 6,758 results for: sas

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pEZYeGFP-SARS-CoV-2E GGG

Plasmid

#224582

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E GGG (E SARS-CoV-2)

Tags

GFP

Expression

Mammalian

Mutation

PBM (LLV) mutated to GGG

Promoter

CMV

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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CCND2 targeting gRNA

Plasmid

#215318

Purpose

Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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CCND1 targeting gRNA

Plasmid

#215153

Purpose

Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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FokI(inactive)-mCherry-LacI-NLS

Plasmid

#215100

Purpose

Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FokI(inactive)-mCherry-LacI-NLS

Expression

Mammalian

Mutation

FokI(inactive) mutant D69A.

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBSWHhc-p53

Plasmid

#133903

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (TP53 Mouse)

Expression

Yeast

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-5'3'short

Plasmid

#129750

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/S647A

Plasmid

#74938

Purpose

Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Mutation

S647A (mutation in the D2 domain of the protein)

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
macroH2A1.1-F192V-GFP (pc5114)

Plasmid

#223437

Purpose

Mutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.1-F192V (Macroh2a1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E stop

Plasmid

#224581

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 lacking its PBM in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E stop (E SARS-CoV-2)

Tags

GFP

Expression

Mammalian

Mutation

stop codon before PBM

Promoter

CMV

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro 2xStrep-HPC4-Gb5

Plasmid

#218177

Purpose

Used to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

G beta subunit 5 (GNB5 Human)

Use

Lentiviral

Tags

2x Strep tag and HPC4

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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NEIL3-mNeonGreen

Plasmid

#215144

Purpose

Expresses NEIL3-mNeonGreen in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NEIL3 (NEIL3 Human)

Use

Retroviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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SS-Cyclin D3

Plasmid

#215116

Purpose

Expresses SS-Cyclin D3 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND3 (CCND3 Human)

Tags

SS (STREP-STREP)

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA T7-Gnat1 Mut-3UTR

Plasmid

#184027

Purpose

Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

TAG sites in the 3'-UTR are mutated to TGA

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-POLB-KO-g1

Plasmid

#176090

Purpose

Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

POLB sgRNA #1 (POLB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68 [dRING]

Plasmid

#126549

Purpose

Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

RING domain deletion mutant [dRING mutation is a …

Promoter

LTR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68 [Y252D]

Plasmid

#126550

Purpose

Expresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Y252D point mutant

Promoter

LTR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/T646A

Plasmid

#74937

Purpose

Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Mutation

T646A (mutation in the D2 domain of the protein)

Promoter

CMV

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only
EXO1B-AcGFP

Plasmid

#215125

Purpose

Expresses EXO1B-AcGFP in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

EXO1B (EXO1 Human)

Use

Retroviral

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-Cyclin B1

Plasmid

#215049

Purpose

Expresses EGFP-Cyclin B1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCNB1 (CCNB1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSS02:cyt-Peredox-mCherry_DS

Plasmid

#161747

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry_DS

Expression

Plant

Mutation

amino acid substitution D115S in first T-Rex doma…

Promoter

Ubiquitin 10

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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