Addgene

We narrowed to 17,703 results for: STO

Showing: 9061 - 9090 of 17703 results

Results per page:

Sort results by:

pMSR:MSMEG_0225:Dendra

Plasmid

#159043

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Joseph Wade, Keith Derbyshire

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0225

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSR:MSMEG_0710:Dendra

Plasmid

#159020

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Joseph Wade, Keith Derbyshire

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0710

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSR:MSMEG_0384:Dendra

Plasmid

#159013

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Joseph Wade, Keith Derbyshire

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0384

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSR:MSMEG_0229:Dendra

Plasmid

#159005

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Joseph Wade, Keith Derbyshire

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0229

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSR:MSMEG_0024:Dendra

Plasmid

#158997

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Joseph Wade, Keith Derbyshire

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0024

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
T7pr-His6-MBP-TEV-Cas9-BirA*

Plasmid

#159993

Purpose

Bacterial expression of Cas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

Cas9-BirA*

Use

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Mm_Plk5(300-595) PBD domain

Plasmid

#136316

Purpose

Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

fragment 300-595

Promoter

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SENP2 NPm

Plasmid

#126593

Purpose

Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 1-65 and L329A/L331A in t…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SENP2 CCm

Plasmid

#126594

Purpose

Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 203-228 and siRNA resista…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-KAT14-547-782

Plasmid

#118376

Purpose

Gateway compatible donor vector with KAT14-547-782 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Tags

Expression

Mutation

Truncation (aa 547-782)

Promoter

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

Plasmid

#122450

Purpose

Expression of inositol 1,4,5-trisphosphate 3-kinase A

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Use

Tags

beta-galactosidase

Expression

Bacterial

Mutation

coding sequence from V66 till stop codon

Promoter

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

Plasmid

#117663

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pOst1-pro-af(MUT2)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-KAT14-1-546

Plasmid

#118377

Purpose

Gateway compatible donor vector with KAT14-1-546 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Tags

Expression

Mutation

Truncation (aa 1-546)

Promoter

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-PIG-N3FLAG-TAF12(50-130)

Plasmid

#105595

Purpose

retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

truncation fragments containing aa (50-130), and …

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDC32-FIT2-N[80]A

Plasmid

#96992

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Use

Tags

Expression

Plant

Mutation

Amino acid residue 80 was mutated (N[80]A). Mutat…

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HDAC4*

Plasmid

#71402

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4* (HDAC4 Human)

Use

Tags

Expression

Mammalian

Mutation

After aa126, insertion leads to reading frame shi…

Promoter

CAG

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL4D-V5-His6

Plasmid

#67447

Purpose

Mammalian expression of hArl4D with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4D-V5-His6

Plasmid

#67426

Purpose

Expression of hArl4D with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4D-GFP

Plasmid

#67405

Purpose

Mammalian expression of hArl4D with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBullet-cyt-n

Plasmid

#53065

Purpose

destination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Expression

Mutation

Promoter

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBullet-er-n

Plasmid

#53067

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Expression

Mutation

Promoter

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBullet-tgn-n

Plasmid

#53071

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB-pLAC-L108S/L109S/F112S

Plasmid

#48730

Purpose

Bacterial expression of Lacritin Protein with Mutation at proteins 108 and 109 Leucine to Serine and 112 from phenylalanine to serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Use

Tags

Intein

Expression

Bacterial

Mutation

Leucine 108 and 109 to Serine; phenylalanine 112 …

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a I18A

Plasmid

#40043

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a I18A (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Isoleucine 18 to Alanine

Promoter

CMV promoter

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a linker

Plasmid

#40040

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a linker (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

linker domain (144-353)

Promoter

cmv

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pAS_4xMma PylT_FLAG-AbKRS-chIPYE

Plasmid

#140020

Purpose

Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

Promoter

EF1

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-Sec61b-C1

Plasmid

#90992

Purpose

Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mEmerald-Sec61b (SEC61B Synthetic, Human)

Use

Tags

mEmerald (green fluorescence)

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-pHluorin_M153R-CD63-mScarlet

Plasmid

#172118

Purpose

Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

mScarlet and pHluorin_M153R in a small extracellu…

Expression

Mammalian

Mutation

Promoter

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge