We narrowed to 22,678 results for: Sis;

1. pOAD

   Plasmid

   #196825

   Purpose

   Yeast two-hybrid ; Gal4 activation domain

   Depositor

   Stan Fields

   Article

   Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

   Type

   Empty backbone

   Use

   Tags

   Gal4 Activation domain

   Expression

   Yeast

   Mutation

   Promoter

   ADH

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-Z33

   Plasmid

   #120050

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   FLAG
   

   Use

   Pcr template

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-DUET-1-mRhubarb719-HO

   Plasmid

   #141200

   Purpose

   Expresses mRhubarb719 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb719
   heme oxygenase
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGSTag BimL

   Plasmid

   #24251

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   BimL (BCL2L11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pSicoR mouse Dicer3

   Plasmid

   #14781

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   Dicer shRNA (Dicer1 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-Lyn-AktAR

   Plasmid

   #123356

   Purpose

   FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   Lyn-AktAR
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL1.7XX

   Plasmid

   #49344

   Purpose

   luciferase reporter with mouse Bmp2 promoter

   Depositor

   Melissa Rogers

   Article

   Abrams et al J Biol Chem. 2004 Apr 16;279(16):15916-2

   Insert

   Bmp2 distal promoter (Bmp2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   nt –1237 to +471

   Promoter

   Bmp2 distal promoter

   Available Since

   Dec. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2ADW_2paRAF

   Plasmid

   #129654

   Purpose

   For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-puro-CLIP-FLAG-CD59

   Plasmid

   #50375

   Purpose

   Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   CLIP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. wt1aCreERT2

    Plasmid

    #108464

    Purpose

    Used to generate a cryst:mCherry wt1a:CreERT2 zebrafish transgenic line.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    wt1aCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. RAB14 3'UTR WT

    Plasmid

    #61489

    Purpose

    luciferase reporter containing RAB14 3' UTR

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 3' UTR (RAB14 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    contains position 837-1637 (numbering relative to…

    Promoter

    PGK

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pSC201

    Plasmid

    #129387

    Purpose

    A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.

    Depositor

    Silvia Cardona

    Article

    Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do

    Insert

    oriR6K and oriT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    N/A

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCaSper4 cyto roGFP2-Orp1

    Plasmid

    #64998

    Purpose

    Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMX-PGC1

    Plasmid

    #6

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2005

    Availability

    Academic Institutions and Nonprofits only

15. TRPML1-MYC

    Plasmid

    #18824

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-sCS4-2T

    Plasmid

    #28250

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain Cse4-Scm3-H4 with 2 Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between Cse4 and Scm3. An…

    Promoter

    Available Since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMaCTag-P24

    Plasmid

    #120035

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TAP
    

    Use

    Pcr template

    Tags

    TAP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-scrCdh2.1 GFP

    Plasmid

    #209100

    Purpose

    GFP expressing scramble of shRNA targeting Cdh2

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCdh2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_CMV:Flag-CeNLuc_SV40:PuroR

    Plasmid

    #183036

    Purpose

    Expresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM6-At4CL-CmCHS-CmCHI

    Plasmid

    #73395

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMaCTag-P10

    Plasmid

    #120021

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKOk
    

    Use

    Pcr template

    Tags

    mKOk

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. XE68 APC-GFP in CS2+

    Plasmid

    #16687

    Depositor

    Randall Moon

    Article

    Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

    Insert

    APC-GFP (APC Human)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3-FLAG-MKRN1_H307E

    Plasmid

    #78756

    Purpose

    To overexpress MKRN1_H307E in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H307E

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-mWasabi-ER

    Plasmid

    #164165

    Purpose

    Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJOG603

    Plasmid

    #105356

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    promotor RPS5a (At3g11940; 1700bp)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI/ BpiI restriction sites removed

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMaCTag-P18

    Plasmid

    #120029

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEBG-TFII-I deltaBR

    Plasmid

    #22185

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Deleted amino acids 301-306

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

28. OSCA1.2-PP-GFP

    Plasmid

    #135390

    Purpose

    Expresses OSCA1.2 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

    Insert

    OSCA1.2 (AT4G22120 Mustard Weed)
    

    Use

    Tags

    EGFP tag connected to the gene via a precision pr…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FH95pUp95GW (B4)

    Plasmid

    #74013

    Purpose

    lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

    Depositor

    Robert Malenka, Weifeng Xu, Oliver Schluter

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBS-Foxd3 in situ

    Plasmid

    #37098

    Depositor

    Patricia Labosky

    Article

    Labosky et al Mech Dev. 1998 Aug;76(1-2):185-90.

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only