We narrowed to 11,691 results for: NSI

1. pJMP2

   Plasmid

   #79874

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into amyE

   Depositor

   Carol Gross, Jason Peters, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   veg

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-P-CAG-GFP

   Plasmid

   #80491

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   CAG-GFP
   

   Use

   Donor vector (human)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK172 asyn(1-122)

   Plasmid

   #240598

   Purpose

   Overexpress truncated alpha-Synuclein protein

   Depositor

   Jennifer Lee

   Article

   Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

   Insert

   aSyn (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   aa 1-122 only

   Promoter

   T7

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK172 asyn(1-103)

   Plasmid

   #240597

   Purpose

   Overexpress truncated alpha-Synuclein protein

   Depositor

   Jennifer Lee

   Article

   Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

   Insert

   aSyn (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   aa 1-103 only

   Promoter

   T7

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNeonGreen-C1-Nir2-LNS2

   Plasmid

   #220086

   Purpose

   C-terminal PA binding domain of Nir2

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM1 (PITPNM1 Human)
   

   Tags

   NeonGreen-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 896-1216

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TRE-dCas9-Zim3-MeCP2

   Plasmid

   #220143

   Purpose

   doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

   Depositor

   Frank Buchholz

   Article

   Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

   Insert

   dCas9-Zim3-MeCP2
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   Tre-3G

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEVOL-AckRS

   Plasmid

   #137976

   Purpose

   sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

   Depositor

   Wenshe Liu

   Article

   Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

   Insert

   AckRS and pylTcua
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Cyto-ExRai-AktAR2

   Plasmid

   #184048

   Purpose

   Cytosol-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Cyto-ExRai-AktAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNES-EGFP-C1-flex-PABD-Spo20

   Plasmid

   #220081

   Purpose

   Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   Spo20 (SPO20 Budding Yeast)
   

   Tags

   X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

   Expression

   Mammalian

   Mutation

   amino acids 51-91

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Expression

    Mammalian

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-MTOR-S2215Y

    Plasmid

    #69013

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2215Y

    Promoter

    CMV

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-CRY2clust

    Plasmid

    #105624

    Purpose

    Expresses improved CRY2PHR homo-oligomerization

    Depositor

    Won Do Heo

    Article

    Park et al Nat Commun. 2017 Jun 23;8(1):30. doi: 10

    Insert

    mCherry-CRY2PHR-9aa (CRY2 Mustard Weed, Human)
    

    Tags

    9 amino acids

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid

    #101299

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 and 698-906 of alpha-C…

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-NPC1(WT)-FLAG

    Plasmid

    #164972

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+_hsTRPA1_myc_dTom

    Plasmid

    #183179

    Purpose

    myc tagged hsTRPA1 used for cell transfection

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Inserts

    hsTRPA1 (TRPA1 Human)
    dTom
    

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CDK1as_T2A_puromycin

    Plasmid

    #118595

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP Sec22b

    Plasmid

    #101918

    Purpose

    Expresses Sec22b in mammalian cells

    Depositor

    Thierry Galli

    Article

    Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. do

    Insert

    Sec22b (Sec22b Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Integrin β8-GFP

    Plasmid

    #205093

    Purpose

    Express GFP-tagged human integrin β8 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 8 (ITGB8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiSAM v2 (Puro)

    Plasmid

    #92062

    Purpose

    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (sgRNA) and EF1a (dCas9-VP64)

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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