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pCfb13212

Plasmid

#219887

Purpose

The base plasmid of TUNEYALI for TF22

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF22 (YALI1_C25288g) and homologous arm matching TF22

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13202

Plasmid

#219877

Purpose

The base plasmid of TUNEYALI forTF12

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF12 (YALI1_C06870g) and homologous arm matching TF12

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13234

Plasmid

#219907

Purpose

The base plasmid of TUNEYALI for TF44

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF44 (YALI1_B22561g) and homologous arm matching TF44

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13219

Plasmid

#219894

Purpose

The base plasmid of TUNEYALI for TF29

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF29 (YALI1_C09133g) and homologous arm matching TF29

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13248

Plasmid

#219919

Purpose

The base plasmid of TUNEYALI for TF58

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF58 (YALI1_F37742g) and homologous arm matching TF58

Expression

Yeast

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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UNK_6xZNF_pGEX

Plasmid

#215703

Purpose

Recombinant expression of UNK with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

UNK (UNK Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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Msi1_2xRRM_pGEX

Plasmid

#215704

Purpose

Recombinant expression of mouse Msi1 with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

Musashi-1 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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p202-Syt1-C2A-I248V

Plasmid

#201554

Purpose

Expresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coli

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Synaptotagmin-1 C2A domain (partial)

Expression

Bacterial

Mutation

changed Isoleucine 248 to Valine (numbering based…

Promoter

RBS

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

Plasmid

#201552

Purpose

Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1

Tags

HaloTag

Expression

Insect

Mutation

changed lysine 282 to arginine (numbering based o…

Promoter

Ac5

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p202-Syt1-C2A-wt

Plasmid

#201553

Purpose

Expresses wild-type Octopus synaptotagmin-1 C2A domain in E. coli

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Synaptotagmin-1 C2A domain (partial)

Expression

Bacterial

Mutation

Codon-optimized for E. coli

Promoter

RBS

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 54kDa

Plasmid

#201375

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-4PrA

Expression

Mammalian

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pCAGGS

Plasmid

#206052

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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L_NES 34kDa

Plasmid

#201353

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP-1PrA

Expression

Mammalian

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 54kDa

Plasmid

#201344

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-4PrA

Expression

Mammalian

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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L_NES 47kDa

Plasmid

#201354

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP-3PrA

Expression

Mammalian

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 54kDa

Plasmid

#201371

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP-4PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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L_NLS 54kDa

Plasmid

#201360

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-4PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 27kDa

Plasmid

#201363

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 27kDa

Plasmid

#201364

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 34kDa

Plasmid

#201373

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-1PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge