We narrowed to 6,907 results for: mag

1. pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54)

   Plasmid

   #242671

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + G1104K mutations in SpCas9(G1104K/D1135V/G…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-SpCas9-VRQR(+A61R)-P2A-EGFP (KAC1030)

   Plasmid

   #242665

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + A61R mutations in SpCas9(A61R/D1135V/G1218…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-SpCas9-VRQR(+L244Q)-P2A-EGFP (LRF96)

   Plasmid

   #242667

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + L244Q mutations in SpCas9(L244Q/D1135V/G12…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53)

   Plasmid

   #242678

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + N1317R mutations in SpCas9(D1135V/G1218R/N…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134)

   Plasmid

   #242676

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-ABE8.8m-eVRQR-P2A-EGFP (BKS974)

   Plasmid

   #242655

   Purpose

   CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with neVRQR(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-ABE8.8m-VRQR(S55R)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.8 mutations in TadA, eVRQR mutations in SpCa…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)

   Plasmid

   #242675

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-ABEmax-eVRQR-P2A-EGFP (LLH575)

   Plasmid

   #242654

   Purpose

   CMV promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with neVRQR(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-ABEmax-VRQR-S55R-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE7.10 mutations in TadA, eVRQR mutations in SpC…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

   Plasmid

   #242681

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

    Plasmid

    #242672

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

    Plasmid

    #242664

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VQR mutations in SpCas9(D1135V/R1335Q/T1337R)

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)

    Plasmid

    #242674

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)

    Plasmid

    #242680

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid

    #210391

    Purpose

    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)

    Depositor

    Benjamin Kleinstiver

    Article

    Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:

    Insert

    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CamKII promoter for Cre, human U6 promoter for Sp…

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid

    #238007

    Purpose

    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

    Depositor

    Simon Powell, Agnel Sfeir

    Article

    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

    Insert

    AAVS1 (AAVS1, PPP1R12C Human)
    

    Use

    Rna donor to study rt-dsbr

    Expression

    Mammalian

    Mutation

    3bp insertion signature (ATC) at Cas9 cut site in…

    Promoter

    CMV

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-mEGFP-HMGB1-delFS

    Plasmid

    #194555

    Purpose

    Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del FS" variant

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    truncation by stop codon after Lys183

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-mEGFP-HMGB1-MUT-Rdel

    Plasmid

    #194556

    Purpose

    Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R del" variant

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Deletion of Arginines after Lys185

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-mEGFP-HMGB1-MUT-RorKtoA

    Plasmid

    #194558

    Purpose

    Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R or K > A" variant

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    R or K > A substitutions after Lys185

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-mEGFP-DVL1-MUT

    Plasmid

    #194587

    Purpose

    Mammalian Expression of mEGFP-DVL1 Mutant (H502Pfs*143) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    DVL1 (DVL1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    H502Pfs*143

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-mEGFP-HMGB1-MUT-RtoA

    Plasmid

    #194557

    Purpose

    Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > A" variant

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    R > A substitutions after Lys185

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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