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AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

Plasmid

#91879

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A)

Plasmid

#122986

Purpose

AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT

Depositor

Matthew Kennedy

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

mCh-IRES-SspB(micro)-BoNT/B(147-441)

Use

AAV

Tags

mCh

Expression

Mammalian

Mutation

SspB contains R73Q "micro" mutation, Bo…

Promoter

human synapsin

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-CyPet

Plasmid

#102636

Purpose

In vitro expression of CyPet from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

CyPet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3-CARMA2sh-E138A

Plasmid

#97068

Purpose

Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamic Acid 138 to Alanine

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

Plasmid

#75118

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pJHG003

Plasmid

#126454

Purpose

Expresses Cas9-XTEN-dTdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

Added D343E and D345E to inactivate TdT.

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S293A

Plasmid

#115197

Purpose

Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S293A (PDHA2 Human)

Use

Lentiviral

Mutation

p.S293A

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

Plasmid

#24419

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

RS1 (HTR4 Human)

Use

Mouse Targeting

Tags

FLAG and mCherry

Mutation

Human 5HT4B receptor with D100A mutation, generat…

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

Plasmid

#75116

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

single amino acid mutation N216A

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_BET-PGK-Puro-IRES-GFP

Plasmid

#75120

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the first bromodomain o…

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

Plasmid

#128551

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 ∆cap/∆bund

Plasmid

#74891

Purpose

mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

V689D, L693D, L757A, K759A

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
MmCavin4-APEX2-P2A-mKate2

Plasmid

#170524

Purpose

Vector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJF

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP

Plasmid

#75121

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRG1

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP

Plasmid

#75119

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD1

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
SKM2 X057_pECIA2

Plasmid

#115114

Purpose

Bait vector SKM2 X057_pECIA2 should be used with prey vector SKM2 X057_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G56040 (AT5G56040 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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BAM1 X060_pECIA2

Plasmid

#115117

Purpose

Bait vector BAM1 X060_pECIA2 should be used with prey vector BAM1 X060_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G65700 (BAM1 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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PXC3 X011_pECIA2

Plasmid

#115118

Purpose

Bait vector PXC3 X011_pECIA2 should be used with prey vector PXC3 X011_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G41820 (AT2G41820 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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EFR D5_pECIA2

Plasmid

#115119

Purpose

Bait vector EFR D5_pECIA2 should be used with prey vector EFR D5_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G20480 (EFR Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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