We narrowed to 11,977 results for: pcDNA

1. XE253 pCDNA3.1(zeo)-hDsh3-82-716

   Plasmid

   #16756

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-82-716-Dsh3 (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   aa 82-716

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

   Plasmid

   #157693

   Purpose

   mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1-FLAG-SBP-STAG2 (S97X)

   Plasmid

   #73964

   Purpose

   STAG2 cDNA (CCDS43990) S97X

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   S97X

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3 Flag MFF iso5 S172A

   Plasmid

   #74382

   Purpose

   Flag tagged human S172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3-IgL-C99-T4L-rTA

   Plasmid

   #177840

   Purpose

   Tango assay

   Depositor

   Karsten Melcher

   Article

   Yan et al Acta Pharmacol Sin. 2017 Oct;38(10):1412

   Insert

   C terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

   Plasmid

   #168094

   Purpose

   Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   Nup62-Ubc-Flag-EPEA
   

   Use

   Tags

   BC2-Tag, EPEA-tag, and Flag-tag

   Expression

   Mammalian

   Mutation

   P229A, S283T on Nup62 native sequence

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 Flag MFF iso5 S155,172A

   Plasmid

   #74383

   Purpose

   Flag tagged human S155,172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   See 155 Ala, Ser 172 Ala (numbering based on MFF …

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

   Plasmid

   #172581

   Purpose

   For transient expression of C-terminal BirA-tagged MACROD1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD1 (MACROD1 Human)
   

   Use

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

   Plasmid

   #127308

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4-Tet-on-vsv-Incenp-EGFP

    Plasmid

    #108483

    Purpose

    inducible expression of vsv-INCENP wt-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG-Fos DD T325A T331A

    Plasmid

    #8973

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362D, S374D, T325A, T331A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

12. FLAG-OTUB1 T134R,C91S-pcDNA3.1

    Plasmid

    #118212

    Purpose

    Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 91 to serine and threonine 134 t…

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 830 pcDNA3 GFP PTEN 1-336

    Plasmid

    #10927

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Amino acids 1-336

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. YFP(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid

    #55762

    Purpose

    An amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    YFP(1-158)-gamma-2 (GNG2 Aequorea victoria, Human)
    

    Use

    Tags

    YFP(1-158) was fused to the amino terminus of Gga…

    Expression

    Mammalian

    Mutation

    YFP (1-158) includes a substitution of Met for Gl…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-MAVS-M1A-invitro(KaganE12)

    Plasmid

    #52016

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M1A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 1 to Ala

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-MAVS-G67M-invitro(KaganD100)

    Plasmid

    #52014

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation G67M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Gly 67 to Met

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-MAVS-L62M-invitro(KaganD98)

    Plasmid

    #52013

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation L62M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Leu 62 to Met

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-MAVS-E80M-invitro(KaganD99)

    Plasmid

    #52015

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation E80M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Glu 80 to Met

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-MAVS-M142A-invitro(E13)

    Plasmid

    #52017

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M142A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 142 to Ala

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Nano-lantern(Ca2+)_63/pcDNA3

    Plasmid

    #51986

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only