167,212 results
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Plasmid#229692PurposeTransient expression of EGFP FP4-mito in mammalian cellsDepositorInsertEGFP; Mitochondira FP4 motifs from Listeria ActA
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE17
Plasmid#90359PurposeNFAT - PKC/Ca++ gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterNFATAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-A50I/I997A
Plasmid#111829PurposeVinculin tension sensor with point mutations affecting ability to bind talin and actinDepositorInsertVinculinTS (I997A)
ExpressionMammalianMutationchanged vinculin alanine 50 to isoleucine and iso…PromoterCMVAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human RNF149-3X FLAG
Plasmid#225332PurposeLentiviral expression of human RNF149 with 3x FLAG tag in mammalian cellsDepositorAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya
Plasmid#186980Purposemammalian expression of Cluc-Gluc reporterDepositorInsertCluc, Gluc
UseLuciferaseAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC67841
Plasmid#209512PurposeCloning in complete guide expression cassettes, with Cas12a (D156R)DepositorTypeEmpty backboneUseCRISPRAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1-SIO-stGtACR2-FusionRed
Plasmid#105677PurposeExpresses a soma-targeted GtACR2 in Cre-expressing neuronsDepositorHas ServiceAAV Retrograde, AAV1, and AAV5InsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterhSyn1Available SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB-gDA3m
Plasmid#208707PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent mannerDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3m
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-Rab6A
Plasmid#190171PurposeExpresses HaloTag-Rab6A in mammalian cells.DepositorInsertHaloTag-Rab6A
TagsHaloTagExpressionMammalianAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-v2-TC
Plasmid#194326PurposeAAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai1-RLuc8
Plasmid#140973PurposeEncodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai1-RLuc8 (GNAI1 Human)
UseSynthetic BiologyExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D
Plasmid#69293PurposeGateway entry vector; single gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone
Plasmid#216152PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic exon and C-terminal FLAG
ExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
SGEP
Plasmid#111170PurposemiR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralMutationWTPromoterSFFVAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-CKM
Plasmid#124134PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
cyto-Ruby3-iATPSnFR1.0
Plasmid#102551Purposeexpresses cytoplasmic ATP sensor in mammalian cells with red reference proteinDepositorInsertRuby3-iATPSnFR1.0
TagsRuby3ExpressionMammalianAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-AncBE4max
Plasmid#174164PurposeThis is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.DepositorInsertAncBE4max
UseLentiviralTagsP2A-mCherryAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
JWW-2 human chimeric monoclonal antibody
Plasmid#66749PurposeExpresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPVDepositorInsertsJWW-2 Heavy Chain
JWW-2 Light Chain
ExpressionMammalianPromotermEF1 and rEF1Available SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsPTB1
Plasmid#64925PurposeExpresses V5- and SBP-tagged PTB1 in mammalian cellsDepositorAvailable SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only